摘要
为探究贝莱斯芽孢杆菌DPT-03对花生白绢病菌的综合防控效果,测定了DPT-03菌悬液、无菌发酵液和挥发性气体对白绢病菌菌丝和菌核的抑制性能,并通过盆栽试验和大田试验探究了DPT-03对花生白绢病的防控效果及对花生产量的影响。结果表明,DPT-03菌悬液、无菌发酵液和挥发性气体均能抑制白绢病菌菌丝生长和菌核形成,其中菌悬液的抑制效果优于无菌发酵液和挥发性气体,培养5 d时DPT-03菌悬液对菌丝生长的抑制率为71.39%,20 d时为87.20%,30 d时对菌核形成的抑制率为88.51%。盆栽条件下DPT-03菌悬液处理能显著降低花生白绢病发病率和病情指数,接种白绢病原菌60 d时防效仍为62.50%,在田间试验中播种后90 d防效达到61.83%,且荚果产量较空白对照增加13.88%。综上,贝莱斯芽孢杆菌DPT-03能显著抑制花生白绢病菌菌丝生长和菌核形成,降低花生白绢病的发病率和发病程度,提升花生产量,在白绢病生物防治中具有较好的应用潜力和较高的研究价值。
In order to explore the comprehensive biocontrol efficacy of Bacillus velezensis DPT-03 on Sclerotium rolfsii in peanut,the inhibitory effects of DPT-03 bacterial suspension,sterile fermentation broth and volatile gas on hyphae and sclerotia of Sclerotium rolfsii were measured,and the effects of DPT-03 on Sclerotium rolfsii and peanut yield were explored through pot and field experiments.The results showed that DPT-03 suspension,sterile fermentation broth and volatile gas could inhibit the hyphae growth and sclerotia formation.Moreover,the inhibition effect of DPT-03 suspension was better than that of sterile fermentation broth and volatile gas.The inhibition rate of DPT-03 bacterial suspension on hyphae growth was 71.39%at 5 days,87.20%at 20 days and 88.51%on sclerotia formation at 30days.Under potted conditions,DPT-03 could significantly reduce the incidence rate and disease index of Sclerotium rolfsii.When inoculated with Sclerotium rolfsii,the control effect was still 62.50%at 60 days,and the control effect reached 61.83%at 90 days after sowing in the field experiment,and the pod yield increased by 13.88%compared with the blank control.In conclusion,Bacillus velezensis DPT-03 can significantly inhibit the hyphae growth and sclerotia formation,reduce the incidence rate and severity of Sclerotium rolfsii and increase the yield of peanut,which has good application potential and high research value in biological control of Sclerotium rolfsii.
作者
彭启超
黄德龙
张志鹏
魏浩
吴书凤
吴妍
李俊
邓祖科
王宗抗
PENG Qichao;HUANG Delong;ZHANG Zhipeng;WEI Hao;WU Shufeng;WU Yan;LI Jun;DENG Zuke;WANG Zongkang(Beijing Long Age AMMS Biological Technology Co.,Ltd.,Beijing 101200,China;Shenzhen Batian Ecotypic Engineering Co.,Ltd.,Shenzhen 518105,China)
出处
《河南农业科学》
北大核心
2022年第2期97-103,共7页
Journal of Henan Agricultural Sciences
基金
深圳市科技计划项目(KCXFZ20201221173211033)。
