摘要
背景:水凝胶作为一类组织工程的支架材料,为移植细胞在体内增殖分化提供了良好的物理支持。目的:探讨水凝胶搭载骨髓间充质干细胞对大鼠受损子宫内膜的修复效果。方法:将分离培养的SD大鼠骨髓间充质干细胞进行CM-Dil标记,然后制备成泊洛沙姆407-骨髓间充质干细胞复合物。取30只8周龄SD雌性大鼠随机分为5组:假手术组、模型组、水凝胶组、干细胞组、复合物组,除假手术组外,其余4组建立子宫内膜机械损伤模型,然后每侧宫角分别注射0.2 mL PBS、泊洛沙姆407水凝胶、骨髓间充质干细胞悬液或泊洛沙姆407水凝胶-骨髓间充质干细胞悬液。观察7 d后取出子宫组织,进行苏木精-伊红染色、免疫荧光染色和酶联免疫吸附检测。结果与结论:①与假手术组相比,模型组、水凝胶组、干细胞组与复合物组内膜厚度变薄(P<0.05),其中水凝胶组、干细胞组和复合物组内膜厚度较模型组均有明显改善(P<0.05),复合物组改善最明显,但水凝胶组与干细胞组间差异无显著性意义(P>0.05);②与干细胞组相比,复合物组CM-Dil标记的骨髓间充质干细胞迁移至子宫内膜数量更多(P<0.01),并且两组细胞均具有向子宫内膜间质细胞分化潜能;③与模型组相比,水凝胶组、干细胞组和复合物组子宫内膜组织中白细胞介素1β、白细胞介素6和和肿瘤坏死因子α水平明显下降(P<0.01),其中,干细胞组和复合物组白细胞介素1β、白细胞介素6和肿瘤坏死因子α水平显著低于水凝胶组(P<0.05),干细胞组白细胞介素6和肿瘤坏死因子α水平低于复合物组(P<0.01),但两组白细胞介素1β水平无显著差异(P>0.05);④结果表明,泊洛沙姆407水凝胶搭载骨髓间充质干细胞治疗子宫内膜损伤具有可行性,为修复受损子宫内膜提供新思路。
BACKGROUND:As a scaffold material for tissue engineering,hydrogels provide a good physical support for the proliferation and differentiation of transplanted cells in vivo.OBJECTIVE:To investigate the effect of hydrogel combined with bone marrow mesenchymal stem cells on endometrial reproduction in rats.METHODS:Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated,cultured,and labeled with CM-Dil and were prepared for Pluronic F-127-bone marrow mesenchymal stem cells complex.A total of 30 Sprague-Dawley rats aged 8 weeks old were randomly divided into sham operation group,model group,hydrogel group,stem cell group,and complex group.Except for the sham operation group,endometrial mechanical injury models were established in the other four groups and each uterine horn was injected with 0.2 mL of PBS,Pluronic F-127 hydrogel,bone marrow mesenchymal stem cell suspension,and Pluronic F-127 hydrogel-bone marrow mesenchymal stem cell suspension,respectively.After 7 days of observation,the uterus tissues were removed for hematoxylin-eosin staining,immunfluorescence staining,and enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION:(1)Compared with the sham operation group,the endometrial thickness of the rats was thinner in the model group,hydrogel group,stem cell group,and complex group(P<0.05).Compared with the model group,the endometrial thickness of hydrogel,stem cell,and complex groups was significantly improved(P<0.05).The complex group had the most obvious effect,while there was no significant difference between hydrogel group and stem cell group(P>0.05).(2)Compared with stem cell group,the number of stem cells labeled with CM-Dil migrated to the endometrium was more in the complex group(P<0.01)and both groups had the potential to differentiate into endometrial stromal cells.(3)Compared with the model group,the levels of interleukin-1β,interleukin-6,and tumor necrosis factorαwere significantly decreased in the endometrium of the hydrogel,stem cell,and complex groups(P<0.01).The levels of inter
作者
吕妍
关永格
宋阳
李玥
Lyu Yan;Guan Yongge;Song Yang;Li Yue(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510360,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第31期4940-4945,共6页
Chinese Journal of Tissue Engineering Research
基金
广东省自然科学基金(2021A1515011435),项目负责人:宋阳
广东省自然科学基金(2020A1515010587),项目负责人:关永格
国家自然科学基金(81873331),项目参与人:关永格。
关键词
水凝胶
骨髓间充质干细胞
组织修复
子宫内膜损伤
组织工程
hydrogel
bone marrow mesenchymal stem cell
tissue repair
endometrial injury
tissue engineering
