摘要
Amorpha-4,11-diene synthase(ADS)catalyzes the first committed step in the artemisinin biosynthetic pathway,which is the first catalytic reaction enzymatically and genetically characterized in artemisinin biosynthesis.The advent of ADS in Artemisia annua is considered crucial for the emergence of the specialized artemisinin biosynthetic pathway in the species.Microbial production of amorpha-4,11-diene is a breakthrough in metabolic engineering and synthetic biology.Recently,numerous new techniques have been used in ADS engineering;for example,assessing the substrate promiscuity of ADS to chemoenzymatically produce artemisinin.In this review,we discuss the discovery and catalytic mechanism of ADS,its application in metabolic engineering and synthetic biology,as well as the role of sesquiterpene synthases in the evolutionary origin of artemisinin.
出处
《aBIOTECH》
CSCD
2021年第3期276-288,共13页
生物技术通报（英文版）
基金
the National Natural Science Foundation of China(31872666)
the Special Fund for Talent Introduction of Kunming Institute of Botany,CAS
the China Postdoctoral Science Foundation(Grant Nos.2020M671252 and 2020T130668)
the Young Elite Scien tists Sponsorship Program by CAST(2019QNRC001)
the Open Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources(PFGR201902).
