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野生扁蓿豆种子硬实破除方法研究 被引量:11

Study on the Method of Breaking Hardness of Wild Medicago ruthenica Seeds
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摘要 为探求破除扁蓿豆种子硬实特性的最佳方法,采用砂纸打磨、切割、液氮处理、热水浸种、酸(98%浓硫酸、20%盐酸溶液)、碱(30%氢氧化钠溶液)等7种处理方法,开展了破除扁蓿豆种子硬实的效果研究。结果表明,切割处理能够完全破除种子硬实,种子发芽率达到99.00%;砂纸打磨后种子的硬实率可下降到7.50%,发芽率达到82.50%;100℃热水浸种5 min,发芽率可达77.00%,并且对幼苗伤害较小;浓硫酸处理15~40 min,硬实率下降到3.00%,发芽率均达到90%以上,但处理时间过长会导致萌发后幼苗不能正常生长;液氮处理5~40 min,均能使发芽率提高到60.00%以上,但处理时间过长对幼苗伤害较大;20%盐酸和30%氢氧化钠处理对破除种子硬实效果不显著。 To explorethe best way of breaking the seed hardness of Medicago ruthenica,7 treatment methods including sand paper grinding,cutting,liquid nitrogen processing,hot water soaking,acid(98%H;SO;,20%HCl)and alkali(30%NaOH)were used to break the seed hardness of M.ruthenica.The results showed that cutting treatment could completely break the seed hardness and the germination rate reached 99.00%,followed by the treatment with sand paper grinding,which could be reduced to less than 7.50%and the germination rate reached 82.50%.The treatment with 5 min in 100℃hot water could increase the germination to 77.00%and the damage to the seedlings was small.Sulfuric acid treatment for 15-40 min and the seed hardness rate could be reduced to less than 3.00%,and the germination rate could reach more than 90%,but the treatment with too long time would lead to the seedlings could not grow normally.Liquid nitrogen treatment for 5-40 min could increase the germination rate to more than 60.00%,but the longer the treatment time,the greater damage to seedlings.Finally,the treatment with 20%hydrochloricacid and 30%sodium hydroxide had no significant effect on breaking the seed hardness of M.ruthenica.
作者 伊风艳 杨鼎 温超 晔薷罕 巩青 吴玉霞 布仁朝古拉 张璞进 YI Fengyan;YANG Ding;WEN Chao;YE Ruhan;GONG Qing;WU Yuxia;Buren chaogula;ZHANG Pujin(Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Zhuozi County Agriculture and Animal Husbandry Bureau,Ulanchap Inner Mongolia 012300,China;The Second School Affiliated to Inner Mongolia Normal University,Hohhot 010090,China)
出处 《种子》 北大核心 2022年第3期132-136,144,共6页 Seed
基金 内蒙古自治区自然科学基金项目:直立型扁蓿豆四倍体的诱导研究(2019 BS 03032)。
关键词 扁蓿豆 萌发 种子硬实 Melilotoides ruthenica germination seed hardness
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