摘要
目的 建立复方薄荷脑苯酚搽剂中水杨酸和苯酚定量分析方法。方法 采用紫外-可见分光光度法(UV)测定水杨酸含量;采用高效液相色谱法(HPLC)测定苯酚含量,色谱柱:Agilent Zorbax SB-C_(18)(250×4 mm,5μm),流动相:甲醇-水(48∶52),检测波长:270 nm,进样量:10μL,流速:1.0 mL·min^(-1),柱温:25℃。结果 水杨酸:在0.0051~0.0304 mg·mL^(-1)范围内具有良好的线性关系(回归方程Y=25.4849X+0.0095,r=0.9992),平均加样回收率为100.91%,RSD为0.69%;苯酚:在进样量0.2640~3.2940μg范围内具有良好的线性关系(回归方程Y=940.8260X+4.0051,r=0.9999),平均加样回收率为100.49%,RSD为0.57%。结论 所建立方法简便、结果准确、重复性好,适用于复方薄荷脑苯酚搽剂的质量控制。
OBJECTIVE To establish the quantitative analysis method of salicylic acid and phenol in Compound menthol phenol liniment.METHODS The content of salicylic acid was determined by UV-VIS spectrophotometry.The content of phenol was determined by High-performance liquid chromatography with Agilent Zorbax SB-C_(18) column(250×4 mm,5 μm) as the chromatographic column.The mobile phase consisted of Methanol-water(48∶52).The detection wavelength was 270 nm and the injection volume was 10 μL.The flow rate was 1.0 mL·min^(-1) and the column temperature was 25 ℃.RESULTS The salicylic acid had good linear relation in the concentration range of 0.0051-0.0304 mg·mL^(-1),and the regression equation was Y=25.4849 X+0.0095,r=0.9992.The average sample recovery rate was 100.91% with RSD 0.69%.The phenol had good linear relation in the injection volume range of 0.2640-3.2940 μg,and the regression equation was Y=940.8260 X+4.0051,r=0.9999.The average sample recovery rate was 100.49% with RSD 0.51%.CONCLUSION The estabished method is simple,reliable and reproductive,and could be used for quality control of Compound menthol phenol liniment.
作者
陈燕霞
陈洁
黄樱华
丘振文
李得堂
黄月纯
孙亦群
CHEN Yan-xia;CHEN Jie;HUANG Ying-hua;QIU Zhen-wen;LI De-tang;HUANG Yue-chun;SUN Yi-qun(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)
出处
《海峡药学》
2022年第3期78-81,共4页
Strait Pharmaceutical Journal
基金
广州中医药大学第一附属医院创新强院工程项目(2017ZWB10)。
关键词
复方薄荷脑苯酚搽剂
质量控制
水杨酸
苯酚
含量测定
Compound menthol phenol liniment
Quality control
Salicylic acid
Phenol
Content determination
