摘要
目的·研究烟酰胺单核苷酸(nicotinamide mononucleotide,NMN)对衰老小鼠代谢的影响。方法·采用随机数字表将70只C57BL/6N雄性小鼠随机分为5组,分别为对照组、早衰模型组、干预Ⅰ组、衰老模型组和干预Ⅱ组,每组14只。除对照组外,其余4组小鼠颈背部皮下注射D-半乳糖(D-galactose,D-gal)(150 mg/kg),建立小鼠衰老模型;干预Ⅰ组和干预Ⅱ组同时灌胃给予NMN(300 mg/kg),其余各组给予等量的蒸馏水,均每日1次,早衰模型组和干预Ⅰ组持续6周,衰老模型组和干预Ⅱ组持续12周;对照组给予等量的生理盐水和蒸馏水,每日1次,持续6周。造模6周后检测对照组、早衰模型组、干预Ⅰ组小鼠呼吸代谢量、活动量、能量消耗等能量代谢监测指标,计算胸腺、脾脏、肝脏、肾脏的脏器指数,检测糖耐量和胰岛素敏感性以及血清和肝脏中的超氧化物歧化酶(superoxide dismutase,SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(malondialdehyde,MDA)含量。造模12周后对衰老模型组和干预Ⅱ组进行上述指标的检测。结果·与对照组比较,早衰模型组和衰老模型组小鼠胸腺指数(P=0.035,P=0.000)和肾脏指数(P=0.009,P=0.002)降低,夜晚的O_(2)消耗量(P=0.018,P=0.000)、CO_(2)呼出量(P=0.044,P=0.003)、活动量(均P=0.000)和能量消耗(P=0.010,P=0.001)显著下降,胰岛素敏感性降低(P=0.012,P=0.011),血清SOD(P=0.002,P=0.001)、GSH-Px(P=0.001,P=0.011)活性显著下降,MDA含量显著升高(均P=0.000)。能量代谢监测指标、胸腺和肾脏指数以及抗氧化指标的下降验证了D-gal衰老模型造模成功。与早衰模型组相比,干预Ⅰ组在呼吸代谢、能量消耗、糖耐量和胰岛素敏感性等指标方面差异无统计学意义(均P>0.05),活动量(P=0.022)和血清SOD(P=0.026)、GSH-Px(P=0.006)活性显著升高,MDA含量显著降低(P=0.011)。与衰老模型组相比,干预Ⅱ组夜晚的O_(2)消耗量(P=0.045)、CO_(2)呼出量
Objective·To investigate the effects of nicotinamide mononucleotide(NMN)on metabolism in aging mice.Methods·Seventy C57BL/6N male mice were randomly divided into 5 groups by using a table of random numbers.They were the control group,the premature aging model group,the aging model group,the intervention groupⅠand the intervention groupⅡ.Each group contained 14 mice.Except the control group,D-galactose(D-gal)(150 mg/kg)was subcutaneously injected into the napes of mice in the other 4 groups to establish the aging model of mice.NMN(300 mg/kg)was given to the intervention groupⅠand the intervention groupⅡby intragastric administration at the same time,and the other groups were given the same amount of distilled water,once a day,for 6 weeks in the premature aging model group and the intervention groupⅠ,and for 12 weeks in the aging model group and the intervention groupⅡ.The control group was given the same amount of normal saline and distilled water,once a day,for 6 weeks.Six weeks after modeling,the energy metabolism levels of the mice in the control group,the premature aging model group and the intervention groupⅠwere detected,including respiratory metabolism,activity level and energy consumption.The organ indexes of thymus,spleen,liver and kidney were calculated.The glucose tolerance and insulin sensitivity were measured.In addition,the content of superoxide dismutase(SOD)and the activities of glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in the serum and liver tissue were detected.Twelve weeks after modeling,the above indexes were detected in the aging model group and the intervention groupⅡ.Results·Compared with the control group,the thymus index(P=0.035,P=0.000)and renal index(P=0.009,P=0.002)of the model groups were significantly decreased.The O_(2) consumption(P=0.018,P=0.000),CO_(2) exhalation(P=0.044,P=0.003),energy consumption(P=0.010,P=0.001)and activity ability(both P=0.000)of the premature aging model group and the aging model group were significantly decreased at night.The i
作者
党国栋
洪新宇
蔡美琴
DANG Guodong;HONG Xinyu;CAI Meiqin(School of Public Health,Shanghai Jiao Tong University,Shanghai 200025,China;Shanghai Center for Disease Control and Prevention,Shanghai 200051,China)
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2022年第2期158-165,共8页
Journal of Shanghai Jiao tong University:Medical Science
关键词
烟酰胺单核苷酸
衰老
能量代谢
糖耐量
胰岛素敏感性
抗氧化
nicotinamide nucleotide(NMN)
aging
energy metabolism
glucose tolerance
insulin sensitivity
antioxidation
