摘要
目的:采用Box-Behnken响应面法结合指纹图谱技术优化决明子总蒽醌提取工艺。方法:以综合评价为衡量指标,在单因素的试验基础上,进行三因素三水平的响应面试验。综合评价指标包括决明子总蒽醌提取率或每克决明子提取物相当药材量、每个样品指纹图谱中橙黄决明素等5种指标成分峰面积占16个共有峰峰面积归一化值及对照指纹图谱相似度(以《中国药典》2020年版一部决明子含量测定提取溶剂建立参照图谱),其权重分别为0.2、0.5和0.3。结果:所得最佳提取工艺:液料比为20∶1 mL/g,提取溶剂为60%乙醇-乙酸乙酯(2∶1)混合溶液,提取时间为15.12 min。5组验证实验结果表明,最佳工艺的决明子总蒽醌综合评价为0.528[相对标准偏差(relative standard deviation,RSD)=0.45%],响应面法模型预测结果为0.531,与预测结果的相对误差为0.58%,最佳提取工艺与模型预测结果一致,所得最佳工艺可用于决明子总蒽醌的提取。结论:采用Box-Behnken响应面法结合指纹图谱技术可综合、全面、准确找到最佳的反应条件及灵敏考察各因素之间的相互作用,可为中药提取工艺优化及评价提供参考与借鉴。
Objective:The Box-Behnken response surface method combined with fingerprint was used to optimize the extraction process of total anthraquinone from Cassia seeds.Methods:A three-factor,three-level response surface test was conducted based on the single-factor test with comprehensive evaluation as the measurement index.The comprehensive evaluation indexes included the extraction rate of total anthraquinone of Cassia seeds or the equivalent amount of herbs per gram of total anthraquinone of Cassia seeds,the normalized value of peak areas of 5 index components such as Aurantio obtusin in the fingerprint of each sample to 16 shared peaks and the similarity of fingerprints(the reference fingerprint was established by the extraction solvent for the determination of Cassia seeds content in the Chinese Pharmacopoeia 2020 edition)with the weights of 0.2,0.5 and 0.3,respectively.Results:The best extraction process was obtained:the liquid-to-material ratio was 20∶1(mL/g),the extraction solvent was mixture of 60%ethanol-ethyl acetate(2∶1),and the extraction time was 15.12 min.The results of five sets of validation experiments showed that the overall evaluation of total anthraquinone of Cassia seeds by the best process was 0.528(relative standard deviation,RSD=0.45%),and the prediction result of response surface method model was 0.531,and the relative error with the prediction result was 0.531.The relative error of the predicted results was 0.58%,and the best extraction process was consistent with the model prediction,and the obtained best process could be used for the extraction of total anthraquinone from Cassia seeds.Conclusion:The Box-Behnken response surface method combined with the fingerprint technique can be used to find the best reaction conditions and examine the interactions among the factors in a comprehensive and accurate manner,which can provide reference for the optimization and evaluation of the extraction process of Chinese medicine.
作者
王恒
邹纯才
鄢海燕
WANG Heng;ZOU Chun-cai;YAN Hai-yan(Pharmacy School of Wannan Medical College,Wuhu 241002,China)
出处
《海南医学院学报》
CAS
2022年第7期507-515,共9页
Journal of Hainan Medical University
基金
2018年度安徽省省级质量工程项目(2018jyxm1273)
安徽高校省级自然科学研究重大项目(KJ2016SD60)
2021年安徽省大学生创新创业计划项目。
