摘要
【目的】明确植物次生代谢物对甜菜夜蛾Spodoptera exigua生长发育及解毒酶的影响,探索利用植物次生物质防控甜菜夜蛾的潜在途径。【方法】本研究选用3种含量(0.01%、0.1%和1.0%)的槲皮素、山奈酚和香豆素,分别与人工饲料混合均匀后饲养甜菜夜蛾3龄初幼虫,观察植物次生代谢物对幼虫生长发育的影响;并测定幼虫取食添加0.1%的槲皮素、山奈酚和香豆素的人工饲料24、48和72 h后,幼虫羧酸酯酶(Caboxylesterase,CarE)、谷胱甘肽-S-转移酶(Glutathione-S-transferase,GSTs)和P450解毒酶活性。【结果】添加不同次生物质的人工饲料显著影响甜菜夜蛾幼虫生长和解毒酶活性。与对照组相比,3种次生代谢物均显著提高了幼虫死亡率。幼虫取食添加1%槲皮素的人工饲料后,蛹重显著降低,发育历期明显延长。而取食添加0.1%山奈酚的人工饲料后,可诱导幼虫CarE活性显著增强,0.1%槲皮素和0.1%香豆素对幼虫CarE活性均有显著抑制作用。添加槲皮素对幼虫GSTs活性无显著性影响,添加0.1%山奈酚和0.1%香豆素可诱导幼虫GSTs活性显著升高。0.1%槲皮素和0.1%香豆素可促进幼虫P450活性增强但未达到显著水平,但0.1%山奈酚处理48 h后,幼虫P450活性显著降低。【结论】植物次生代谢物种类与含量对甜菜夜蛾生长发育及解毒酶活性存在不同程度的影响。
[Objectives]To determine the effects of plant secondary metabolites on the growth,development and detoxification enzyme activity,of Spodoptera exigua,and investigate the potential of using these substances to control this pest.[Methods]Three different concentrations(0.01%,0.1%and 1.0%)of three different plant secondary compounds,quercetin,kaempferol or coumarin,were added to the artificial diet fed to 3rd instar S.exigua larvae and their growth and development were observed and compared.In addition,the activity of caboxylesterase,glutathione S-transferase and cytochrome P450,were measured 24,48 and 72 h after larvae had been treated with 0.1%quercetin,kaempferol or coumarin.[Results]All three secondary metabolites significantly increased larval mortality.The pupal weight of larvae treated with of 1.0% quercetin was significantly less than that of the control group,whereas their developmental duration was obviously longer.CarE activity was significantly higher in larvae treated with 0.1% kaempferol,but 0.1% quercetin and 0.1% coumarin significantly inhibited CarE activity.Although quercetin had no significant effect on GSTs,0.1% kaempferol and 0.1% coumarin increased GSTs.The P450 activity of larvae treated with 0.1% quercetin or 0.1% coumarin was higher than that of the control group,although not significantly so,whereas the P450 activity of larvae treated with 0.1% kaempferol was significantly lower than that of the control group after 48 hours.[Conclusion]Different plant secondary metabolites have different effects on the growth and detoxification enzyme activity of S.exigua larvae.
作者
范能能
王金彦
万年峰
蒋杰贤
FAN Neng-Neng;WANG Jin-Yan;WAN Nian-Feng;JIANG Jie-Xian(College of Life Science and Fishery,Shanghai Ocean University,Shanghai 201306,China;Eco-environmental Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2022年第1期165-171,共7页
Chinese Journal of Applied Entomology
基金
国家自然科学基金面上项目(31672083)
上海市农业科学院卓越团队建设项目(农科创2018(B-01))。
