摘要
目的研究热应激致HSPA2表达下调的内在机制。方法通过构建大鼠生殖系统的热应激模型,并运用MSP和BSP检测大鼠睾丸组织中Hspa2基因启动子区CpG岛的甲基化状况。结果MSP:无论是使用甲基化引物,还是非甲基化引物,热应激组和对照组都能成功扩增出目的DNA片段,在所研究的区域内,两组DNA样本均同时存在甲基化与非甲基化两种状态。BSP:热应激组和对照组各含20个有效单克隆,440个CpG位点,其中热应激组发生甲基化的CpG位点总数为6,对照组发生甲基化的CpG位点总数为7,热应激组和对照组在BSP所扩增的目标DNA片段内,其甲基化率没有明显差异(P>0.05)。结论热应激在短时间内导致HSPA2的表达下调,可能不是通过DNA的甲基化修饰来实现的。此结论还需进一步研究证实。
Objective To study the internal mechanisms of HSPA2 expression reduction due to heat stress.Methods The heat stress model of the reproductive system of rats was constructed,and MSP and BSP were used to detect methylation of CpG Island in the Hspa2 gene promoter region in the testicular tissue of rats.Results MSP:Whether using methylation primers or non-methylation primers,the heat stress group and the control group were able to successfully amplify the target DNA fragments,and in the area studied,the DNA samples of the heat stress group and the control group were present in both methylation and non-methylation.BSP:The heat stress group and the control group each contained 20 effective monoclonal,440 CpG sites,of which the total number of CpG sites where methylation occurred in the heat stress group was 6,the total number of CpG sites with methylation in the control group was 7,and the methylation rate of the heat stress group and the control group was not significantly different in the target DNA fragments amplified by BSP(P>0.05).Conclusion Heat stress leads to a reduction in the expression of HSPA2 in a short period of time,which may not be achieved by methylation modification of DNA.this conclusion needs further study to confirm.
作者
李锐
陈雪丹
张庆华
Li Rui;Chen Xuedan;Zhang Qinghua(Reproductive Medicine Center,Daping Hospital,Army Medical University,Chongqing 400042,China;Department of Medical Genetics,College of Basic Medical Science,Army Medical University(Third Military Medical University),Chongqing 400038,China)
出处
《中国男科学杂志》
CAS
CSCD
2022年第1期38-42,共5页
Chinese Journal of Andrology
基金
重庆市技术创新与应用示范项目(社会民生类重点研发项目)(cstc2018jscx-mszd X0020)
军队后勤科研计划延续项目计生专项(19JSZ02)。
