摘要
为了研究核转运蛋白SsKapJ在甘蔗鞭孢堆黑粉菌中发挥的作用,本研究利用PEG介导的原生质体转化方法获得基因敲除突变体,并进行了相关生物学表型分析。结果显示潜在核转运蛋白基因SsKapJΔ突变体有性配合和菌丝生长显著减弱,转录组分析SsKapJ参与环磷酸腺苷/蛋白激酶A(cAMP/PKA)和丝裂原活化蛋白激酶(MAPK)信号途径调控。另一方面,SsKapJΔ突变体担孢子对吡唑醚菌酯杀真菌剂敏感性增强,但突变体对苯菌灵杀真菌剂敏感性以及环境胁迫测试(渗透压、细胞壁抑制剂、氧化还原和乙醇)中较野生型没有明显变化。本研究结果揭示了SsKapJ核转运蛋白在甘蔗鞭孢堆黑粉菌的有性配合和菌丝生长过程中具有重要调控作用,为后续开展核运输途径调控真菌有性配合/二态型转变研究提供基础。
In order to study the role of karyopherin SsKapJ in the sugarcane smut fungus Sporisorium scitamineum,the deletion mutant was generated by using the PEG-mediated protoplast transformation method,and the mutant phenotypes were characterized.The results showed that the putative karyopherin gene deletion mutant SsKapJDdisplayed a significant reduction in sexual mating and filamentation.Transcriptome analysis revealed that SsKapJ was involved in regulation of cyclic adenosine monophosphate/protein kinase A(cAMP/PKA)and mitogen-activated protein kinase(MAPK)signaling pathway.The haploid sporidia of SsKapJD mutant was hypersensitive to pyraclostrobin fungicide,but no difference was found in sensitivity towards benomyl fungicide or the tested stressful conditions(osmotic pressure,cell wall,redox and ethanol)as compared with the wild-type.This study suggests that SsKapJ karyopherin may play an important regulatory role in sexual mating and filamentation of S.scitamineum,providing a basis for further study on regulation of fungal dimorphic switch via nuclear transport pathway.
作者
陈金辉
甘永健
崔国兵
邓懿祯
CHEN Jinhui;GAN Yongjian;CUI Guobing;DENG Yizhen(College of Plant Protection,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrate Microbiology Research Center,South China Agricultural University,Guangzhou 510642,Guangdong,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第3期478-491,共14页
Mycosystema
基金
广州市科技计划重点项目:甘蔗鞭黑穗病生物防治技术的研发与应用(201904020010)。
