摘要
【背景】狐、貉肺炎频发导致养殖户遭受巨大经济损失。【目的】调查黑龙江省、吉林省、河北省、山东省等地狐、貉肺炎原因,对病原菌进行分离、鉴定及部分生物学特性研究。【方法】无菌采集患病狐、貉肺组织进行细菌分离培养;对分离菌进行革兰氏染色、生化试验、特异性基因PCR鉴定、药物纸片扩散法敏感性试验、毒力基因检测及小鼠致病性试验。【结果】分离的136株菌的革兰氏染色均为阴性杆菌,生化特性及特异性基因PCR均鉴定为大肠杆菌;分离株具有多重耐药性且有29种毒力基因组合方式,其主要流行毒力基因有8种:yijP、fimC、fimH、fyuA、iutA、papC、irp2、astA;小鼠致病性结果显示,分离菌株含主要流行毒力基因越多对小鼠致病性越强。【结论】明确了引发狐、貉肺炎的病原菌为大肠杆菌,其携带毒力基因数量与致病力相关。
[Background]Frequent occurrence of pneumonia in foxes and raccoon dogs has resulted in huge economic losses for farmers.[Objective]In order to investigate the causes of pneumonia attacking foxes and raccoon dogs in Heilongjiang,Jilin,Hebei,and Shandong provinces,we isolated,identified,and characterized the pathogenic bacteria.[Methods]The bacteria were isolated aseptically from the lung tissues of diseased foxes and raccoon dogs.Gram staining,biochemical test,PCR identification of specific genes,susceptibility test by disk diffusion method,virulence gene detection,and mouse pathogenicity test were then conducted for the isolates.[Results]All the 136 isolates were Gram-negative bacilli and were identified as Escherichia coli based on biochemical characteristics and specific gene PCR.The isolates had multiple drug resistance and 29 virulence gene combinations.The main virulence genes were yijP,fimC,fimH,fyuA,iutA,papC,irp2,and astA.The isolates carrying more virulence genes had stronger pathogenicity to mice.[Conclusion]E.coli was identified as the pathogen causing pneumonia of foxes and raccoon dogs,and the number of virulence genes was related to the pathogenicity.
作者
李倩倩
靳元元
赵强
李峰
尉发正
任洪林
胡盼
王晓旭
LI Qianqian;JIN Yuanyuan;ZHAO Qiang;LI Feng;YU Fazheng;REN Honglin;HU Pan;WANG Xiaoxu(Key Laboratory of Zoonosis Research,Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun 130062,Jilin,China;Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,Jilin,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2022年第3期1048-1056,共9页
Microbiology China
基金
中央级公益性科研院所基本科研业务费专项(Y2021XK23)。
关键词
狐、貉肺炎
大肠杆菌
耐药性
毒力基因
致病性
fox and raccoon dog pneumonia
Escherichia coli
drug resistance
virulence gene
pathogenicity
