摘要
研究西瓜抗感病毒病种质间的遗传多样性和亲缘关系,以提高西瓜抗病毒病新品种选育效率。本试验从50对SSR引物中筛选出12对多态性高且稳定的引物,对51份抗感病毒病西瓜种质进行简单重复序列标记(SSR)分析,共获得187个稳定且清晰的条带,其中多态性条带142个,多态率可达75%。供试西瓜种质的观测等位基因数(Na),有效等位基因数(Ne),Nei’s基因多样性指数(H)和Shannon’s信息指数(I)分别为1.7594、1.2899、0.1854、0.2961。供试西瓜种质的遗传相似系数的范围在0.50~0.94之间,遗传差异系数小,表明SSR分子标记能够区别遗传变异小的种质。以遗传系数0.78为界,UPGMA聚类可将51份供试西瓜种质分为5大类,抗病毒病型西瓜种质主要集中在第I、Ⅱ、Ⅲ大类,感病型西瓜种质主要集中在第Ⅲ、Ⅳ、Ⅴ大类,野生抗病毒病西瓜种质均分布在第I大类,表明抗病毒病型野生西瓜种质与其他种质间的亲缘关系最远。Cophentic相关性检验的遗传相似性原始数据矩阵与UPGMA聚类结果矩阵相关系数值R为0.825,说明SSR分子标记能够准确地分析供试西瓜种质间的差异。供试抗病毒型西瓜种质的遗传多态性高于感病型西瓜种质,供试西瓜种质总体的遗传多样性不够丰富,要拓展西瓜抗病毒病种质资源的遗传基础,需要加强遗传差异大的新种质的收集和利用。
The genetic diversity and relationship among antiviral and susceptible watermelon germplasms were studied in order to improve the breeding efficiency of new varieties of antiviral watermelon.In this experiment,12pairs of primers with high polymorphism and stability were selected from 50 pairs of SSR primers,and 51 antiviral and susceptible watermelon germplasms were analyzed by Simple Sequence Repeat(SSR).A total of 187 stable and clear bands were obtained,of which 142 were polymorphic,and the polymorphism rate was up to 75%.The observed number of alleles(Na),the effective number of alleles(Ne),the diversity index of Nei’s gene(H)and the Shannon’s information index(I)of the tested watermelon germplasms were 1.7594,1.2899,0.1854,0.2961 respectively.The genetic similarity coefficient of the tested watermelon germplasms ranged from 0.50 to 0.94,and the coefficient of genetic difference was small,which indicated that SSR molecular markers could distinguish the germplasms with small genetic variation.With the genetic coefficient of 0.78 as the boundary,51 watermelon germplasms could be divided into 5 categories by UPGMA clustering.Type of antiviral watermelon germplasms mainly concentrated in the I,Ⅱ,Ⅲcategories,type of susceptible watermelon germplasms mainly concentrated in theⅢ,Ⅳ,Ⅴcategories,and the wild antiviral watermelon germplasms were distributed in theIcategory,which indicated that the wild antiviral watermelon germplasms and other germplasms showed distantly related.The cophentic correlation test showed that the genetic similarity raw data matrix and UPGMA clustering results matrix relationship value(R)was 0.825,which indicated that SSR molecular markers could accurately analyze the differences among the tested watermelon germplasms.The genetic polymorphism of antiviral watermelon germplasms was higher than that of susceptible watermelon germplasms,and the genetic diversity of the tested watermelon germplasms was not abundant.In order to expand the genetic basis of antiviral watermelon germpla
作者
高宁宁
李晓慧
康利允
常高正
梁慎
徐小利
李海伦
王慧颖
赵卫星
Gao Ningning;Li Xiaohui;Kang Liyun;Chang Gaozheng;Liang Shen;Xu Xiaoli;Li Hailun;Wang Hui-ying;Zhao Weixing(Horticulture Institute,Henan Academy of Agricultural Sciences,Zhengzhou,450002)
出处
《分子植物育种》
CAS
北大核心
2022年第4期1239-1249,共11页
Molecular Plant Breeding
基金
国家西甜瓜产业技术体系建设项目(CARS-25)
河南省重点研发与推广专项(192102110036)、河南省重点研发与推广专项(202102110041)
河南省农业科学院自主创新资助项目(2021ZC19)共同资助。
