摘要
研究以γ-Fe_(2)O_(3)为基础的磁性纳米微球通过化学修饰是否可以具有过氧化物模拟酶的催化性质,通过制备SiO_(2)包被的γ-Fe_(2)O_(3)磁性纳米粒子,采用共价修饰方法对γ-Fe_(2)O_(3)@SiO_(2)磁性纳米粒子用葡萄糖氧化酶(GOD)修饰后得到γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD磁性纳米微球,通过红外吸收光谱(IR)对γ-Fe_(2)O_(3)、γ-Fe_(2)O_(3)@SiO_(2)、γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)、γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH和γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD磁性纳米微球进行结构确证。结果表明,磁性纳米微球的氨基化、羧基化及GOD修饰成功。利用荧光分光光度计测定后,证明γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD具有过氧化物模拟酶的催化性质,可以催化H_(2)O_(2)和TMB之间的反应,葡萄糖的分解浓度过高酶也会出现淬灭现象。
This article intends to study whetherγ-Fe_(2)O_(3)-based magnetic nanoparticles can have the catalytic properties of peroxidase mimics by chemical modification.By preparing SiO_(2)-coatedγ-Fe_(2)O_(3) magnetic nanoparticles,and then adopting covalent modification methods,γ-Fe_(2)O_(3)@SiO_(2) magnetic nanoparticles were modified with glucose oxidase(GOD),γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD magnetic nanoparticles were obtained,and thenγ-Fe_(2)O_(3),γ-Fe_(2)O_(3)@SiO_(2),γ-Fe_(2)O_(3)@SiO_(2)-NH_(2),γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH andγ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD magnetic nanoparticles were used for structural confirmation.The results showed that the amination,carboxylation and GOD-modification of the magnetic nanoparticles were successful.After measuring with a fluorescence spectrophotometer,γ-Fe_(2)O_(3)@SiO_(2)-NH_(2)-COOH-GOD was proved to have the catalytic properties of a peroxidase mimicking enzyme,which can catalyze the reaction between H_(2)O_(2) and TMB.However,if the glucose decomposition concentration is too high,the enzyme quenching will also occur.
作者
杨娇
秦铭杉
吕海军
颜雪涛
杨贵军
杨琳燕
Yang Jiao;Qin Mingshan;LüHaijun;Yan Xuetao;Yang Guijun;Yang Linyan(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Key Laboratory of Biological Feed Additive Enterprises,Tianjin 300383,China)
出处
《天津农学院学报》
CAS
2022年第1期47-51,共5页
Journal of Tianjin Agricultural University
基金
国家自然科学基金(31572492,31372482)
天津农学院杰出科研业绩奖励资助项目(无编号)
天津市教委科研计划项目(2017KJ190)
天津市“兽医生物技术创新团队”资助项目(TD13-5091)
广西师范大学省部共建药用资源化学与药物分子工程国家重点实验室资助课题(CMEMR2016-B12)
天津市大学生创新创业训练计划项目(202010061066)。
