摘要
目的:探讨益气补肾调脂方对非酒精性脂肪性肝炎的保护作用及其作用机制。方法:体外培养L02肝细胞,H;O;构建氧化应激模型,设立对照组、模型组、药物组和抑制剂组。CCK8检测各组细胞的存活率,流式细胞仪检测各组细胞的凋亡水平、细胞内活性氧(ROS)水平和线粒体膜电位,ELISA法检测各组细胞内丙二醛(MDA)、乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、还原型谷胱甘肽(GSH)的含量,Western Blot检测各组细胞内Nrf2信号通路相关蛋白的表达水平。结果:益气补肾调脂方药液处理细胞,可显著提高氧化应激模型中细胞的存活率(P<0.01),降低凋亡、ROS水平、线粒体膜电位及MDA、LDH、ALT、AST含量(P<0.05,P<0.01),增加GSH含量及Nrf2蛋白的表达水平(P<0.01)。结论:益气补肾调脂方通过活化Nrf2信号通路,调节CD36的表达,改善非酒精性脂肪性肝炎。
Objective: To study the protection effect of Yiqi Bushen Tiaozhi Decoction on nonalcoholic steatohepatitis,and to investigate the possible mechanism. Methods: L02 cells were cultured in vitro, and building the oxidative stress model induced by H;O;, divided into control group, model group, drug group and inhibitor group. CCK8 was used to measure the survival rate of cells. The apoptosis, intracellular reactive oxygen species(ROS) level and mitochondrial membrane potential were detected by flow cytometry. ELISA kit was used the measure the contents of malondialdehyde(MDA), lactate dehydrogenase(LDH), alanine aminotransferase(ALT), aspartate aminotransferase(AST) and reduced glutathione(GSH). Western Blot was used to measure the protein expression of Nrf2 pathway. Results: Yiqi Bushen Decoction could significantly increase the survival rate of cells(P<0.01), decreased the apoptosis, the level of ROS, mitochondrial membrane potential, and the contents of MDA, LDH,ALT, AST(P<0.05, P<0.01), increased the content of GSH and protein expression of Nrf2(P<0.01). Conclusion: Yiqi Bushen Tiaozhi Decoction could activate the Nrf2 pathway, regulate the expression level of CD36 and protect the live cells in nonalcoholic steatohepatitis.
作者
何蓓晖
罗敏敏
王潇
严茂祥
陈芝芸
HE Bei-hui;LUO Min-min;WANG Xiao;YAN Mao-xiang;CHEN Zhi-yun(The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2022年第2期725-729,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省自然科学基金项目(No.LY17H290007)。
