摘要
目的:观察小柴胡汤对大鼠慢性氟中毒致肝损伤的治疗作用,探讨小柴胡汤在对抗氧化应激中的保护作用,为氟中毒的防治提供理论依据。方法:将SD大鼠随机分为空白对照组、模型组、小柴胡汤实验组和小柴胡汤阴性对照组。以NaF溶液(100 mg/L)为氟源,建立慢性氟中毒大鼠模型。小柴胡汤实验组、小柴胡汤阴性对照组大鼠给予小柴胡汤中药汤液(7.29 g/kg)灌胃,其余组给予等剂量0.9%氯化钠溶液灌胃。持续灌胃90 d(1日1次)后,禁食12 h后将大鼠麻醉,腹主动脉取血,冰浴下解剖取出肝脏组织,计算肝脏指数,肝组织病理形态变化用苏木精-伊红染色法检测;采用微板法检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平,硫代巴比妥酸法检测肝脏丙二醛(MDA)水平,微板法检测肝脏总超氧化物歧化酶(T-SOD)水平。结果:与空白对照组相比,模型组大鼠T-SOD、ALT、AST和MDA水平显著升高,肝脏指数均明显变大,差异均有统计学意义(P<0.01);模型组大鼠病理切片结果显示大量炎性浸润和点状坏死。与模型组相比,小柴胡汤实验组大鼠ALT、AST水平均降低(P<0.05),MDA、T-SOD水平明显降低(P<0.01),肝脏指数均明显减小(P<0.01),差异均有统计学意义;小柴胡汤实验组大鼠病理切片结果显示肝脏病理损伤均有不同程度改善,表明小柴胡汤能改善大鼠慢性氟中毒致肝损伤所引起的病理学变化。结论:小柴胡汤对大鼠慢性氟中毒致肝损伤具有一定的缓解作用,且其作用机制可能与氧化应激相关。
OBJECTIVE:To observe the therapeutic effects of Xiaochaihu decoction for liver injury induced by chronic fluorosis in rats,and to explore the protective effects of Xiaochaihu decoction on oxidative stress,so as to provide theoretical basis for the prevention and treatment of fluorosis.METHODS:SD rats were randomly divided into the blank control group,model group,Xiaochaihu decoction experimental group and Xiaochaihu decoction negative control group.The rat model of chronic fluorosis was established with NaF solution(100 mg/L)as fluoride source.The Xiaochaihu decoction experimental group and the negative control group were given Xiaochaihu decoction(7.29 g/kg)by intragastric administration,while other groups were treated with 0.9% sodium chloride solution by intragastric administration.After 90 d of continuous intragastric administration(once a day),the rats were anesthetized after fasting for 12 h,and the abdominal aorta blood was taken.The liver tissue was dissected under the ice bath,and the liver indicator was calculated.The pathological changes of liver tissue were detected by hematoxylin-eosin staining.Serum ALT and AST levels were detected by microplate method,liver MDA level was detected by thiobarbituric acid method,and liver T-SOD level was detected by microplate method.RESULTS:Compared with the blank control group,the levels of T-SOD,ALT,AST and MDA in the model group increased significantly,and the liver indicators increased significantly,with statistically significant differences(P<0.01).Pathological sections showed a large number of inflammatory infiltrations and dot necroses in the model group.Compared with the model group,the levels of ALT and AST in the Xiaochaihu decoction experimental group decreased(P<0.05),the levels of MDA and T-SOD decreased significantly(P<0.01),and the liver indicators decreased significantly(P<0.01),with statistically significant differences.Pathological section results showed the pathological damage of liver in rats from Xiaochaihu decoction experimental group was improv
作者
陈伟杰
吴浪浪
彭玖燕
梁超男
贺廷贵
吴楚珍
唐文超
CHEN Weijie;WU Langlang;PENG Jiuyan;LIANG Chaonan;HE Tinggui;WU Chuzhen;TANG Wenchao(The Second Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 500025,China;The First Clinical Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 500025,China;College of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 500025,China)
出处
《中国医院用药评价与分析》
2022年第3期277-280,285,共5页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
国家级大学生创新创业项目(No.202010662020)
贵州省中医药管理局中医药、民族医药科学技术课题(No.QZYY-2021-010)。
关键词
小柴胡汤
氟中毒
氧化应激
肝损伤
Xiaochaihu decoction
Fluorosis
Oxidative stress
Liver injury
