摘要
目的通过探讨m6A去甲基化酶FTO基因DNA甲基化与乙肝疫苗免疫低/无应答水平的关联性,为预测个体乙肝疫苗的应答水平、研发新型HepB疫苗提供科学的依据。方法采用非匹配病例对照的研究方法,招募于2014-2016年间至广西南宁市三所三级甲等医院就诊的8~9月龄263例儿童(已完成乙肝免疫接种程序)作为研究对象,根据乙肝表面抗体(HBsAb)水平将抗-HBs浓度<100 mIU/mL作为观察组,抗-HBs浓度≥100 mIU/mL作为对照组。采用Methyl Target技术检测FTO基因位点的DNA甲基化水平,分析FTO基因28个位点CpG岛DNA甲基化水平与乙肝疫苗免疫应答水平的关系。结果FTO基因各基因位点的DNA甲基化水平都比较低;观察组和对照组的DNA甲基化水平在FTO_1基因222位点、FTO_2基因72位点的差异具有统计学意义;观察组FTO_1基因222位点的DNA甲基化水平高于对照组(Z=-2.38,P=0.009)。观察组FTO_2基因72位点的DNA甲基化水平高于对照组(Z=-1.71,P=0.044)。结论提示人体中FTO基因存在发生甲基化的可能,乙肝疫苗接种者低/无免疫应答水平可能受FTO_1基因222位点、FTO_2基因72位点的影响。
Objective The research aimed to investigate the relationship between DNA methylation of m6A demethylase FTO gene and low/no response level of hepatitis B vaccine(HepB),and to provide scientific basis for predicting individual response level of HepB and developing new HepB.Methods A total of 263 children aged8 to 9 months(who had completed hepatitis B immunization program)were recruited as subjects in a non-matched case-control study from 2014 to 2016.According to the level of hepatitis B surface antibody(HBsAb),anti-HBs concentration<100 mIU/mL was taken as the case group,and anti-HBs concentration≥100 mIU/mL was taken as the control group.DNA methylation level of FTO gene locus was detected by Methyl Target technique,and the relationship between DNA methylation level of CpG island at 28 locus of FTO gene and immune response level of HepB was analyzed.Results The DNA methylation level of FTO gene loci was low.The differences of DNA methylation levels at FTO_1 gene locus 222 and FTO_2 gene locus 72 were statistically significant between the case group and control group.The level of DNA methylation at 222 locus of FTO_1 gene in case group was higher than that in control group(Z=-2.38,P=0.009).The level of DNA methylation at locus 72 of FTO_2 gene in case group was higher than that in control group(Z=-1.71,P=0.044).Conclusion It was suggested that methylation of FTO gene might occur in human body,and the low/non-immune response level of HepB might be affected by FTO_1 gene locus 222 and FTO_2 gene locus 72.
作者
李嘉铃
周小婷
董柏青
陈钦艳
胡莉萍
王超
苏永健
李海
LI Jialing;ZHOU Xiaoting;DONG Baiqing;CHEN Qinyan;HU Liping;WANG Chao;SU Yongjian;LI Hai(Department of Epidemiology,School of Public Health and Management,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2022年第4期468-472,共5页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81760604,81360443,81703283)
广西自然科学基金项目(编号:2013GXNSFBA019194,2017GXNSFBA198086)
广西中医药大学研究生教育创新计划资助项目(编号:YCSZ2020020,YCXJ2021030)。
