摘要
该文对小鼠结肠类器官培养方法进行了分析总结,旨在建立成熟的结肠类器官培养试验方法,优化培养条件,提高小鼠结肠类器官的生长率,建立稳定的类器官病理检测鉴定方法。以8周龄左右的C57BL/6小鼠为样本来源进行取材,取约5cm长结肠片段,分离结肠隐窝并进行培养,观察结肠类器官形成。结果发现,结肠片段的截取、破碎以及消化时间等多个步骤均为实验的关键点,很大程度上会影响类器官培养效率。该文结合实际操作经验对这些关键的技术步骤进行了归纳总结,并给予相应处理方案,建立并优化了小鼠结肠类器官的培养条件,为结肠类器官的体外建模可行性提供了技术支持。
This paper analyzes and summarizes the culture methods of mouse colon organoids, aiming to establish a mature colon organoid culture method, optimize culture conditions, increase the growth rate of mouse colon organoids, and establish astable pathological detection and identification method for organoids. Take about 5 cm long colon fragments from 8 weeks old C57BL/6 mouse, isolate colonic crypts and culture them to observe the formation of colon organoids. The results show that multiple steps such as the interception, fragmentation, and digestion time of colon fragments are the key points of the experiment, which will greatly affect the efficiency of organoid culture to a great extent. This article summarizes these key technical steps in combination with actual operating experience, and gives corresponding treatment plans, establishes and optimizes the culture conditions of mouse colon organoids, and provides technical support for the feasibility of in vitro modeling of colon organoids.
作者
黄慧茜
毕锋
唐秋琳
HUANG Huiqian;Bi Feng;TANG Qiulin(Laboratory of Molecular Target Theropy in Oncology,West China Hospital of Sichuan University,Chengdu 610041,China)
出处
《实验技术与管理》
CAS
北大核心
2022年第2期18-22,共5页
Experimental Technology and Management
基金
四川大学华西医院学科卓越发展1·3·5工程项目(ZYGD18005)。
