摘要
目的探讨早期非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中补体C3和C4表达水平与老年肥胖患者临床病理特征及预后的价值。方法收集行手术的Ⅰ-Ⅱa期NSCLC患者(年龄≥50岁)120例。采用免疫组织化学法和Western Blot法,检测NSCLC癌和癌旁组织中C3和C4蛋白的表达水平。于术后对患者进行24个月电话随访或门诊随访。结果与癌旁组织相比,低、中、高BMI三组癌组织中C3和C4蛋白水平逐渐增高,且差异具有统计学意义(P<0.05)。相关性分析结果表明,C3和C4免疫组织化学水平与肿瘤分化程度(C3:γ=0.688,P<0.001;C4:γ=0.577,P<0.001)和BMI(C3:γ=0.603,P<0.001;C4:γ=0.590,P<0.001)呈线性关联,且C3和C4蛋白在低分化和高BMI组中水平增高最显著。相比低C3组与低C4组,高C3组(χ^(2)=17.564,P<0.001)和高C4组χ^(2)=9.767,P<0.001)累积生存率均显著降低。随着BMI增高,患者的累积生存率逐渐降低(χ^(2)=14.879,P<0.001)。结论早期NSCLC癌组织中C3和C4蛋白水平与患者分化程度和BMI相关,且高C3和C4蛋白水平均提示NSCLC患者预后较差。
Objective To investigate the correlation of complement C3 and C4 expression with the clinicopathological features and prognosis in elderly obese patients with early non-small cell lung cancer(NSCLC).Methods A total of 120 patients with stage I-IIa NSCLC who underwent NSCLC surgery were enrolled.The expression levels of C3 and C4 proteins in NSCLC and adjacent tissues were detected by immunohistochemistry and western blot.Patients were followed up for 24 months by telephone or outpatient.Results Compared with the adjacent tissues,the levels of C3 and C4 proteins in the low,middle and high BMI tissues increased gradually(P<0.05).Correlation analysis showed that the immunohistochemical levels of C3 and C4 were correlated with the stages of differentiation(C3:γ=0.688,P<0.001;C4:γ=0.577,P<0.001)and BMI C3:γ=0.603,P<0.001;C4:γ=0.590,P<0.001),and the levels of C3 and C4 proteins were most significant in patients with poorly differentiated and high BMI groups.Compared with the low C3 group and the low C4 group,the cumulative survival rate of the high C3 group(χ^(2)=17.564,P<0.001)and the high C4 group(χ^(2)=9.767,P<0.001)was significantly lower.As the BMI increased,the cumulative survival rate of patients gradually decreased(χ^(2)=14.879,P<0.001).Conclusion The levels of C3 and C4 proteins in early NSCLC tissues are associated with patient differentiation and BMI,and high C3 and C4 protein levels suggest a poor prognosis in patients with NSCLC.
作者
芦翼飞
任中海
王磊
贾彦巍
LU Yifei;REN Zhonghai;WANG Lei(Nanyang Central Hospital,Nanyang,473000)
出处
《实用癌症杂志》
2022年第3期367-370,375,共5页
The Practical Journal of Cancer
基金
河南省科技攻关项目(编号:LHGJ20191450)。
