摘要
目的:探讨脐针通过调节肠道菌群及色氨酸代谢在腹泻型肠易激综合征(diarrhea-type irritable bowel syndrome,IBS-D)治疗上的作用机制。方法:选择30例符合罗马Ⅳ标准的IBS-D患者入组,予脐针治疗,疗程4周。征集30例健康志愿者作基线检测对照。观察健康志愿者及IBS组患者治疗前后的临床症状评分,检测粪便肠道菌群,检测血清色氨酸(TRP)、5-羟色胺(5-HT)、犬尿氨酸(KYN)及吲哚2,3-双加氧酶1(IDO1)浓度,以及结肠色氨酸羟化酶1(TPH1)蛋白表达。结果:经脐针治疗后,IBS患者临床症状评分较治疗前降低(P<0.01);肠道菌群多样性增加,结构差异明显;IBS患者血清TRP、5-HT、KYN、IDO1浓度较前下降(P<0.01);结肠TPH1蛋白表达上升(P<0.05)。结论:脐针治疗能改善IBS-D患者临床症状,其作用机制可能与通过调节肠道菌群及色氨酸代谢,抑制5-HT及KYN生成相关。
Objective: To investigate the mechanism of Umbilicus acupuncture on diarrhea-type irritable bowel syndrome(IBS-D) based on gut microbiota and tryptophan metabolism. Methods: Thirty patients with IBS-D who met the Rome Ⅳ diagnostic criteria for IBS-D were enrolled and treated with Umbilicus acupuncture for 4 weeks. Thirty healthy volunteers served as baseline controls. The changes of clinical symptom scores before and after treatment were observed. Gut microbiota were also detected. And serum tryptophan(TRP), 5-hydroxytryptamine(5-HT), kynurenine(KYN), as well as indoleamine 2, 3-dioxygenase1(IDO1) were recorded. The colonic tryptophan hydroxylase1(TPH1) protein expression was detected before and after treatment. Results: The clinical symptom scores of IBS patients after treatment in IBS group were lower than those before treatment(P<0.01). The diversity of gut microbiota was increased, and significant differences were in gut microbiota structure. The levels of TRP, 5-HT, KYN and IDO1 were also decreased(P<0.01). Colonic TPH1 expression levels were increased after treatment(P<0.05). Conclusion: Umbilicus acupuncture can improve the clinical symptoms of IBS-D patients, and its mechanism of action may be related to the inhibition of 5-HT and KYN production by regulating gut microbiota and tryptophan metabolism.
作者
俞蕾敏
吴婵妮
叶蔚
张娅丽
范一宏
YU Leimin;WU Channi;YE Wei;ZHANG Yali;FAN Yihong(Department of Gastroenterology,Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou,310007,China;Department of Gastroenterology,Zhejiang Xiaoshan Hospital;Department of Gastroenterology,First Affiliated Hospital of Zhejiang Chinese Medical University)
出处
《中国中西医结合消化杂志》
CAS
2022年第3期211-216,共6页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
浙江省中医药科技计划项目(No:2021ZB200)
浙江省医药卫生科技计划项目(No:2021KY917)。
关键词
腹泻型肠易激综合征
脐针
肠道菌群
色氨酸代谢
diarrhea-type irritable bowel syndrome
Umbilicus acupuncture
gut microbiota
tryptophan me-tabolism
