摘要
目的研究miRNA-125a-5p对胰腺癌皮下移植瘤细胞凋亡的影响。方法使用miRNA-125a-5p过表达、低表达慢病毒稳定转染PANC-1细胞。选取BALB/C雄性裸鼠18只,随机分为3组,每组6只,分别接种miRNA-125a-5p过表达载体、低表达载体的稳转株细胞和胰腺癌PANC-1细胞,分为miRNA-125a-5p过表达组、miRNA-125a-5p低表达组和对照组(PANC-1组)。最后收集皮下移植瘤,RT-qPCR检测各组miRNA-125a-5p mRNA及凋亡相关因子Caspase-8mRNA表达;Western Blot检测凋亡相关因子Caspase-8蛋白表达水平。结果miRNA-125a-5p过表达组miRNA-125a-5p mRNA表达较对照组明显升高,miRNA-125a-5p低表达组miRNA-125a-5p mRNA表达较对照组明显降低,差异有统计学意义,提示转染效果好。miRNA-125a-5p过表达组Caspase-8mRNA及蛋白表达较miRNA-125a-5p低表达组及对照组明显升高,差异均有统计学意义;miRNA-125a-5p低表达组Caspase-8mRNA及蛋白表达较对照组明显降低,差异有统计学意义。结论miRNA-125a-5p通过促进凋亡相关因子Caspase-8表达,从而调控胰腺癌皮下移植瘤细胞凋亡。
Objective To study the effect of miRNA-125a-5p on the apoptosis of subcutaneously transplanted tumor cells of pancreatic cancer.Methods PANC-1 cells were transfected with lentivirus of miRNA-125a-5p over-expression and those of miRNA-125a-5p low-expression.Eighteen BALB/C male mice were randomly divided into three groups with 6 mice in each group.These mice were inoculated with stable transgenic cells transfected by over-expressed miRNA-125a-5p,with those transfected by low-expressed miRNA-125a-5p and with PANC-1 cells,respectively.And they were divided into over-expressed miRNA-125a-5p group,low-expressed miRNA-125a-5p group and the control group(PANC-1 group).Finally,subcutaneously transplanted tumors were collected,and the miRNA expressions of miRNA-125a-5p and Caspase-8(apoptosis-related factor)were detected by RT-qPCR.Western Blot was used to detect the protein expression of the apoptosis-related factor Caspase-8.Results The miRNA expression of the over-expressed miRNA-125a-5p group was significantly higher than that of the control group.The miRNA expression of the low-expressed miRNA-125a-5p group was significantly lower than that of the control group,and the difference was statistically significant,suggesting that the transfection effect was good.The miRNA and protein expressions of Caspase-8 in the over-expressed miRNA-125a-5p group were significantly higher than those of low-expressed miRNA-125a-5p group and the control group,with statistically significant difference.The miRNA and protein expressions of Caspase-8 in the low-expressed miRNA-125a-5p group were significantly lower than that in the control group,and the difference was statistically significant.Conclusion miRNA-125a-5p regulates the apoptosis of subcutaneously transplanted tumor cells of pancreatic cancer by promoting the expression of the apoptosis-related factor Caspase-8.
作者
潘路娟
梁华益
刘东菊
周青宏
郑频
覃月秋
Pan Lujuan;Liang Huayi;Liu Dongju;Zhou Qinghong;Zheng Pin;Qin Yueqiu(Department of Gastroenterology,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)
出处
《右江民族医学院学报》
2022年第1期16-19,24,共5页
Journal of Youjiang Medical University for Nationalities
基金
广西自然科学基金项目(2017GXNSFAA198030)
广西医学高层次骨干人才培养计划资助(桂卫科教发〔2018〕22号)
右江民族医学院附属医院2020年度高层次人才科研项目资助(右医附院院字〔2020〕117号)。
