摘要
目的探讨妊娠期糖尿病(GMD)孕妇白细胞介素(IL)-1β和IL-10的基因多态性。方法选取2018年2月至2021年6月在该院建档分娩的488例孕妇作为研究对象,根据GMD发生情况分为发生GMD(GDM组)18例,未发生GMD(非GDM组)470例,检测两组孕妇血清IL-1β和IL-10水平及基因多态性情况,并进行相关性分析。结果GDM组孕妇血清IL-1β和IL-10水平均高于非GDM组,差异均有统计学意义(P<0.05)。两组孕妇IL-1βrs231362和IL-10 rs2283194位点符合Hardy-Weinberg平衡。GDM组孕妇IL-1βrs231362位点CC基因型与C等位基因频率、IL-10 rs2283194位点TT基因型与T等位基因频率与非GDM组比较,差异均有统计学意义(P<0.05)。Spearman相关分析结果显示,GDM与IL-1βrs231362位点CC基因型与C等位基因、IL-10 rs2283194位点TT基因型与T等位基因均存在相关性(P<0.05)。非条件多因素Logistic回归分析结果显示,IL-1βrs231362位点CC基因型与C等位基因、IL-10 rs2283194位点TT基因型与T等位基因均为GMD发生的影响因素(P<0.05)。结论GDM孕妇多伴随有血清IL-1β和IL-10水平高表达,IL-1β和IL-10基因多态性为GDM发生的影响因素。
Objective To investigate the interleukin(IL)-1βand IL-10 gene polymorphism in women with gestational diabetes mellitus(GMD).Methods A total of 488 pregnant women who delivered in the hospital from February 2018 to June 2021 were selected as the research objects.According to the occurrence of GMD,they were divided into 18 cases with GMD(GMD group)and 470 cases without GMD(non-GMD group).The levels and gene polymorphism of serum IL-1βand IL-10 in the two groups of pregnant women were detected,and correlation analysis was carried out.Results The serum IL-1βand IL-10 levels of pregnant women in the GDM group were higher than those in the non-GDM group,and the differences were statistically significant(P<0.05).The IL-1βrs231362 and IL-10 rs2283194 loci of the two groups were consistent with Hardy-Weinberg equilibrium.There were significant differences in the frequency of CC genotype and C allele at IL-1βrs231362 loci,and the frequency of TT genotype and T allele at IL-10 rs2283194 loci between the GDM group and the non-GDM group(P<0.05).Spearman correlation analysis showed that there were correlations between GDM and IL-1βrs231362 loci CC genotype and C allele,and IL-10 rs2283194 loci TT genotype and T allele(P<0.05).Unconditional Multivariate Logistic regression analysis showed that CC genotype and C allele at IL-1βrs231362 loci,and TT genotype and T allele at IL-10 rs2283194 loci were influencing factors affecting the occurrence of GMD(P<0.05).Conclusion Women with GDM are mostly accompanied by high levels of serum IL-1βand IL-10,and IL-1βand IL-10 gene polymorphism are influencing factors for the occurrence of GDM.
作者
林凤
张利娜
LIN Feng;ZHANG Lina(Department of Obstetrics,Weinan Maternal and Child Health Hospital,Weinan,Shaanxi 714000,China)
出处
《检验医学与临床》
CAS
2022年第6期775-778,共4页
Laboratory Medicine and Clinic
