摘要
为探索重楼属不同抗寒力的7种植物的叶片解剖结构特征与其抗寒性的相关性,探究重楼属植物的抗寒机制,以自然生长期(6月)和越冬期(1月)的7种重楼叶片为材料,制作石蜡切片和透射电镜切片,分别观测10项叶肉解剖结构和栅栏细胞超微结构.采用聚类分析法筛选两个时期的抗寒性评价指标,结合隶属函数法对不同重楼抗寒性进行综合评价.结果表明:重楼叶片上下表皮为长方形单层细胞,栅栏细胞1-2层,7种重楼的10项叶片结构指标差异性显著(P<0.05).聚类分析筛选出两个时期的重楼属植物抗寒性评价指标大致相同.其中自然生长期筛选出的指标为叶片总厚度(TL)、细胞结构疏松度(SR)、叶脉突起度(VPD)、栅海比(P/S)和下表皮厚度(TLE);越冬期的抗寒性指标为海绵组织厚度(TS)、栅栏组织厚度(TP)、VPD、P/S和TLE.隶属函数值计算出两个时期的排序结果显示,除长柱重楼排序差别较大外,其余种大致相同.对比两个时期栅栏细胞超微结构表明,低温会导致细胞壁破损,细胞核皱缩,叶绿体中类囊体降解,基粒片层空隙增大,嗜锇颗粒增加,淀粉粒合成受阻,线粒体数量增加.7种重楼的抗寒性强弱排序为越南重楼>皱叶重楼>长柱重楼>宽瓣球药隔重楼>西畴重楼>平伐重楼>云南重楼.抗寒性强的重楼属植物的叶片特征表现为叶片下表皮和栅栏组织更厚,海绵组织更薄,具有更大的细胞组织紧密度以及更大的叶脉厚度和叶脉突起度,细胞核和叶绿体结构更稳定,低温环境下受损程度更小.
The purpose of this study was to explore the correlation between the leaf anatomical structure characteristics of seven Paris species and the degree of their cold resistance,and to explore the cold resistance mechanism of the Paris genus.Paraffin sections and transmission electron microscopy sections were prepared from the leaves of seven Paris species during the natural growth period(June)and overwintering period(January)to observe the anatomical structures of the mesophyll and the ultrastructures of palisade cells,respectively.The cluster analysis method was used to screen the evaluation indices of cold resistance in the two periods,combined with the membership function method to comprehensively evaluate the cold resistance of the different species of Paris.The results showed that the upper and lower epidermis of the leaves of the seven Paris species were rectangular monolayer cells,and the palisade cells were mono-or bilayered.The microstructure of the leaves of the different Paris species was significantly different(P<0.05).The evaluation indices of cold resistance of the Paris genus in the two periods were determined to be similar through cluster analysis.Among them,the indices used for evaluation of cold resistance in the natural growth period were leaf thickness(TL),spongy ratio(SR),vein protuberance degree(VPD),palisade tissue/spongy tissue(P/S),and thickness of lower epidermis(TLE).The indices used for evaluation of cold resistance in the overwintering period included sponge tissue thickness(TS),palisade tissue thickness(TP),VPD,P/S and TLE.Ranking of the results of the two periods calculated by the membership function values shows that except for the large difference in the ranking of P.forrestii,the observed parameters in other species were roughly similar.The comparison of the ultrastructure of palisade cells between the two periods showed that exposure to low temperatures causes cell wall damage,nuclear shrinkage,thylakoid degradation in chloroplasts,enlarged space of grana lamella,increased osmiophi
作者
高驰
赵灵
王庆鹤
任梓萱
赵家雯
尹鸿翔
GAO Chi;ZHAO Ling;WANG Qinghe;REN Zixuan;ZHAO Jiawen;YIN Hongxiang(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第1期111-121,共11页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(81573545)
成都市科技局技术创新研发项目(2019-YF05-00346-SN)资助。
关键词
重楼属
叶片解剖结构
叶片超微结构
抗寒性
综合评价
genus Paris
leaf anatomic structure
leaf ultrastructure
cold resistance
comprehensive assessment
