摘要
胞外多糖是假单胞菌生物被膜的重要组成部分,能增强菌体对外界环境、抗菌剂和宿主防御的耐受性。假单胞菌能产生3种与生物被膜形成密切相关的核心胞外多糖:褐藻胶、Psl和Pel,它们在细菌细胞中的合成和转运分别依赖对应的褐藻胶、Psl和Pel生物合成系统。因此,本综述系统全面地总结了假单胞菌3种胞外多糖生物合成系统结构生物学的研究进展,重点围绕已知结构的蛋白质进行具体的结构和功能阐述。在此基础之上,对未来研究方向提出新的思路和见解,可为假单胞菌生物被膜形成机制及控制策略奠定扎实的理论基础。
Extracellular polysaccharides serve as the major structural components of Pseudomonas biofilm matrix, which can enhance the tolerance of bacteria for environment, antimicrobials and host defense.Pseudomonas mainly produce three key extracellular polysaccharides implicated in biofilm formation: alginate,Psl and Pel, and their synthesis and transport depend on the corresponding alginate, Psl and Pel biosynthetic systems. Therefore, this review comprehensively summarizes the progress on structural biology of Pseudomonas biofilm formation-related three exopolysaccharide biosynthetic systems, and describes the known structures and functions of proteins in these three systems. On this basis, new insights are put forward for future research directions, which can lay a solid theoretical foundation for formation mechanism and control strategies of Pseudomonas biofilm.
作者
刘佳文
吴倩
张昭寰
童金蓉
黄振华
刘静
潘迎捷
赵勇
LIU Jia-Wen;WU Qian;ZHANG Zhao-Huan;TONG Jin-Rong;HUANG Zhen-Hua;LIU Jing;PAN Ying-Jie;ZHAO Yong(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Laboratory of Quality&Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China;Shanghai Engineering Research Centre of Aquatic-Product Processing&Preservation,Shanghai 201306,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2022年第2期273-283,共11页
Progress In Biochemistry and Biophysics
基金
国家博士后创新人才支持计划(BX20190194)
国家自然科学基金(32001800)
上海市“超级博士后”激励计划(2019348)
中国博士后科学基金(2019M661469)资助项目。
