摘要
目的:探讨伤科九味健骨片对家兔骨短缩延长牵拉成骨的作用和血清骨钙素浓度及骨组织BMP2、FGF2表达的影响。方法:选取60只新西兰清洁级家兔,建立单侧胫骨干缺损模型,随机分成3组,空白组采用外固定支架固定,对照组采用胫骨短缩延长术,实验组在短缩延长的基础上予以伤科九味健骨灌胃。分别于胫骨短缩延长术后1周、3周及5周时比较各组家兔骨组织HE染色、Masson染色、血清骨钙素浓度及骨组织BMP2、FGF2的表达水平。结果:胫骨短缩延长术后1周、3周及5周时,组织形态学观察显示,3组均可见新生血管,大量成骨细胞和成软骨细胞;实验组纤维性骨痂均比对照组更成熟,骨小梁排列更加规则,髓腔再通更快;与空白组、对照组比较,实验组在胫骨短缩延长术后3周及5周时,血清骨钙素浓度及骨组织BMP2的基因表达、蛋白表达水平显著提高,实验组在胫骨短缩延长术后1周、3周及5周时,骨组织FGF2的基因表达、蛋白表达水平显著提高,差异有统计学意义(P<0.05)。结论:伤科九味健骨片可促进骨短缩延长牵拉成骨,其机制可能与促进血管、骨组织再生,提高血清骨钙素浓度及骨组织BMP2、FGF2表达水平有关。
Objective:To investigate the efficacy of Jiuwei Jiangu tablet on bone shortening,lengthening and distraction osteogenesis in rabbits,serum osteocalcin concentration and the expression of BMP2 and FGF2 in bone tissue.Methods:60 male and female clean New Zealand rabbits were selected to establish a model of unilateral tibia shaft defect and randomly divided into three groups.The blank group was fixed with external fixation scaffold,the control group was treated with tibia shortening and lengthening,and the experimental group was given Jiuwei jiangu gavage on the basis of shortening and lengthening.HE staining,Masson staining,serum osteocalcin concentration and the expression levels of BMP2 and FGF2 in bone tissue of rabbits in each group were compared at 1,3 and 5 weeks after tibia shortening and lengthening.Results:Histological observation showed that new blood vessels and a large number of osteoblasts and chondroblasts were observed in three groups at 1,3 and 5 weeks after tibia shortening and lengthening.The fibrous callus in the experimental group was more mature than the negative control group,the arrangement of bone trabecula was more regular,and the medullary cavity recanalization was faster.Compared with the blank group and control group,the concentration of serum osteocalcin and bone tissue BMP2 gene expression and protein expression level in experimental group increased significantly at 3 and 5 weeks after tibia shortening and lengthening.The expression level of Bone tissue FGF2 gene and protein increased significantly in experimental group at 1,3 and 5 weeks after tibia shortening and lengthening.The difference was statistically significant(P<0.05).Conclusion:Jiuwei Jiangu tablet can promote bone shortening and elongation,and the mechanism may be related to the promotion of blood vessel and bone tissue regeneration,increasing the concentration of serum osteocalcin and the expression levels of BMP2 and FGF2 in bone tissues.
作者
位春巍
陈嘉怡
郑臣校
WEI Chunwei;CHEN Jiayi;ZHENG Chenxiao(Graduate School of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Hospital of Traditional Chinese Medicine of Zhongshan,Zhongshan 528400,Guangdong China.)
出处
《中国中医骨伤科杂志》
CAS
2022年第2期1-7,共7页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
广东省中医药局科研项目(20191357)
中山市社会公益科技研究项目(2018B1084)。
关键词
骨短缩
牵拉成骨
机制
骨钙素
骨形态发生蛋白
成纤维细胞生长因子
shortening of the bone
traction osteogenesis
mechanism
osteocalcin
bone morphogenetic protein
fibroblast growth factor
