摘要
【目的】通过优化动态超高压微射流法对滇黄精多糖提取的相关工艺条件,分析其结构与活性,为滇黄精多糖的多元化利用提供技术支持。【方法】以滇黄精多糖得率为考察指标,在单因素试验的基础上设计响应面法分析试验,确定动态超高压微射流法提取滇黄精多糖的最佳条件;并对滇黄精多糖的功能活性(酪氨酸酶抑制活性、α-糖苷酶抑制活性及DPPH自由基清除能力等)与结构特性(红外光谱、相对分子量及单糖组成等)进行研究。【结果】经响应面分析构建滇黄精多糖得率的二次回归方程:Y=19.55+0.7A+0.78B+0.89C+0.28AB+1.26AC+0.84BC-1.84A^(2)-4.72B^(2)-2.3C^(2)(R^(2)=0.9860)(Y表示滇黄精多糖得率,A、B、C分别表示微射流压力、料液比和提取时间),提取时间、料液比及微射流压力与提取时间的交互作用对滇黄精多糖得率有极显著影响(P<0.01),微射流压力及提取时间与料液比的交互作用对滇黄精多糖得率有显著影响(P<0.05),3个因素对多糖得率影响的排序为:提取时间>料液比>微射流压力;滇黄精多糖的最佳提取工艺条件为:微射流压力146 MPa、料液比1∶41、于100℃下提取70 min,在此条件下,滇黄精多糖得率为19.57%,达理论预测值的98.69%。滇黄精多糖是一种由11种单糖组成的吡喃型酸性多糖,相对分子量为35.95 kD,具有较强的DPPH自由基清除能力、α-葡萄糖苷酶抑制活性和酪氨酸酶抑制活性,半抑制质量浓度(IC50)分别为0.43、4.07和2.87 mg/mL。【结论】采用响应面法优化动态超高压微射流法提取滇黄精多糖的得率高,预测性良好,滇黄精多糖具有良好的功能活性,有广泛的开发前景。
【Objective】The extraction of polysaccharides from Polygonatum kingianum Coll.et Hemsl.(PKP)by dynamic high-pressure microfluidization(DHPM)were optimized and its structure and activity were analyzed,which provide technical support for the diversified utilization of PKP.【Method】Response surface methodology(RSM)was designed on the basis of single factor experiment to determine the optimal extraction conditions of PKP to determine the optimal extraction conditions by DHPM,with PKP yield as the indicators.The activities(tyrosinase inhibitory activity,α-glycosidase inhibitory activity,DPPH radical scavenging activity)and structural characteristics(infra-red spectrum,relative molecular weight,monosaccharide composition)of PKP were studied.【Result】The quadratic regression equation established by RSM and the model had good fitting degree,as follow:Y=19.55+0.7A+0.78B+0.89C+0.28AB+1.26AC+0.84BC-1.84A^(2)-4.72B^(2)-2.3C^(2)(R^(2)=0.9860)(Y was extraction yield,A was microfluidization pressure,B was materialliquid ratio and C was extraction time).Influence of extraction time,material-liquid ratio and interaction of microfluidization pressure and extraction time to PKP yield were extremely significant(P<0.01).Influence of microfluidization pressure and interaction of material-liquid ratio and extraction time to polysaccharides yield were and significant(P<0.05).Parameters influencing PKP yield were in the order as follows:extraction time>material-liquid ratio>microfluidization pressure.The optimal parameters were microfluidization pressure of 146 MPa,material-liquid ratio of 1∶41,extracting time of 70 min at 100℃.Then the polysaccharides yield was 19.57%,and 98.69%of the theoretical prediction was obtained.PKP was an acidic polysaccharide composed of 11 monosaccharides with a relative molecular weight of 35.95 kD.PKP showed strong DPPH radical scavenging ability,α-glycosidase inhibitory activity and tyrosinase inhibitory activity.The semi-suppressed mass concentration(IC50)were 0.43,4.07 and 2.87 mg/mL respe
作者
梁朋光
孙健
岳健
苏娟
唐雅园
栾会燕
邱福荣
杨兆杏
向昱
何雪梅
LIANG Peng-guang;SUN Jian;YUE Jian;SU Juan;TANG Ya-yuan;LUAN Hui-yan;Khoo Hock Eng;YANG Zhao-xing;XIANG Yu;HE Xue-mei(Yantai Gold Vocational College,Yantai,Shandong 264000,China;Agro-food Science and Technology Rescarch Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Fruits and Vegetables Storage processing Technology,Nanning 530007,China;Yunnan Shanlihong Biotechnology Co.,Ltd,Kunming 650200,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2021年第12期3434-3445,共12页
Journal of Southern Agriculture
基金
云南省科技人才和平台计划项目(2019IC054)
广西科技基地和人才专项(桂科AD19110141)
广西农业科学院基本科研业务费项目(桂农科2021YT112,桂农科2021YTI16)。
关键词
滇黄精多糖
响应面分析
动态超高压微射流
提取工艺
结构
活性
Polygonatum kingianum Coll.et Hemsl.polysaccharides
response surface methodology
dynamic highpressure microfluidization
extraction technique
structure
activity
