摘要
目的:观察软骨膜中细胞初级纤毛消融对长骨生长板发育的影响。方法:雄性4周龄野生型小鼠20只,随机分为对照组和实验组,每组10只。实验组按照0.01 mL/g体质量于小鼠股骨生长板外局部注射10 mg/mL水合氯醛(CH),对照组于相同部位注射等量生理盐水,每2 d注射一次,连续注射4周后处死小鼠。Micro-CT扫描分析软骨下骨矿化情况;行组织学切片观测软骨膜细胞初级纤毛发生率及生长板细胞形态功能变化。结果:实验组小鼠软骨膜中初级纤毛减少,软骨下骨矿化降低;软骨膜中细胞初级纤毛发生受到CH抑制后,可导致股骨生长板软骨细胞排列和功能受损,生长板结构紊乱,生长板下骨小梁数量下降;免疫荧光染色显示实验组软骨膜中细胞初级纤毛发生率下降(P<0.001),且生长板软骨细胞Ⅱ型胶原蛋白分泌功能下降。结论:软骨膜中初级纤毛参与生长板软骨形成和骨骼发育。
To observe the effect of cell primary cilia ablation in femur perichondrium on growth plate development of long bone.Methods:Twenty male 4-week-old wild-type mice were randomly divided into the control group and the experimental group.For the experimental group,chloral hydrated(CH)(0.01 mL/g)was topically injected outside the femur growth plate every 2 days in mice,while the same amount of saline was given in the control group.After 4 weeks of continuous injection,mice were sacrificed.Micro-CT scan was used to analyze the mineralization of subchondral bone,and the incidence of primary cilia and growth plate cell morphology was observed by histological slicing.Results:In the experimental group,the presence of primary cilia in the perichondrium was decreased and the mineralization of subchondral bone was reduced.The formation of primary cilia in the femoral perichondrium was inhibited by CH,which could hinder the arrangement and function of the growth plate chondrocytes,disarrange the structure of the growth plate and decrease the number of bone trabeculae under the growth plate.Immunofluorence staining showed that the incidence of primary cilia in the perichondrium was decreased in the experimental group(P<0.001)and the secretion of typeⅡcollagen of the growth plate chondrocytes was also decreased.Conclusions:Primary cilia in perichondrium was involved in the development of cartilage and bone of the growth plate.
作者
耿江予
陶狄坷
孙瑶
GENG Jiangyu;TAO Dike;SUN Yao(Department of Implantology,School & Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China)
出处
《口腔生物医学》
2022年第1期26-29,44,共5页
Oral Biomedicine
基金
国家自然科学基金优秀青年科学基金(81822012)。
关键词
软骨膜
初级纤毛
生长板
发育
perichondrium
primary cilia
growth plate
bone development
