摘要
目的研究血清维生素D水平和维生素D受体(VDR)基因多态性与肥胖型哮喘儿童的相关性。方法前瞻性分析2016年10月至2019年10月在驻马店市第一人民医院小儿呼吸内科收治的非肥胖型哮喘96例、肥胖型哮喘45例、健康对照组儿童54例,收集儿童的一般临床资料;酶联免疫吸附测定检测外周血中炎性因子白介素(IL)-4、IL-7、IL-10、IL-17、IL-33和25羟维生素D[25(OH)D]水平;检测受试者肺功能指标;全血基因组DNA抽提及检测VDR基因型rs731236、rs1544410、rs1168271和rs2228570位点多态性;logistic回归模型分析影响肥胖型哮喘儿童发病的相关基因型。结果肥胖型哮喘组外周血中炎性因子IL-4、IL-7、IL-10、IL-17和IL-33与对照组比较均差异有统计学意义,肥胖型哮喘组外周血中25(OH)D(18.6±3.2)μg/L低于非肥胖型哮喘组(23.1±3.5)μg/L和对照组(35.6±6.5)μg/L(P<0.05)。三组间第1秒用力呼气容积(FEV1)、用力肺活量(FVC)、一秒率、呼气流量峰值(PEF)和残气量/肺活量(RV/TLV)比较均差异有统计学意义,且肥胖型哮喘组FEV1、一秒率低于非肥胖型哮喘组和对照组,RV/TLV高于非肥胖型哮喘组和对照组,FVC和PEF低于对照组(均P<0.05)。三组儿童VDR基因型rs2228570位点AA、AG、GG基因型和A、G基因频率分布差异有统计学意义。rs2228570位点AA基因型为肥胖型哮喘发病的危险因素(OR=1.365,95%CI:1.024~1.748,P=0.028),GG基因型为保护性因素(OR=0.351,95%CI:0.026~0.514,P=0.002)。结论25(OH)D水平在肥胖型哮喘组中水平降低,VDR基因rs2228570位点AA基因型为肥胖型哮喘的危险因素,GG基因型为保护性因素。
Objective To study the correlation between serum vitamin D level and vitamin D receptor(VDR)gene polymorphism with obese asthmatic children.Methods Prospective analysis was performed of 96 cases of non-obese asthma,45 cases of obese asth⁃ma,who were admitted to Department of Pediatric Respiratory Medicine,Zhumadian First People's Hospital from October 2016 to October 2019,and 54 cases of healthy control children.General clinical data of the children were collected.Enzyme-linked immunosor⁃bent assay was performed to detect the levels of inflammatory factors interleukin(IL)-4,IL-7,IL-10,IL-17,IL-33 and 25-hydroxyvita⁃min D[25(OH)D]in peripheral blood.Pulmonary function indicators of the subjects were tested.Whole blood genomic DNA extraction and detection of vitamin D receptor genotype rs731236,rs1544410,rs1168271 and rs2228570 loci polymorphism were performed.Lo⁃gistic regression model was used to analyze the genotypes associated with asthma in obese children.Results Compared with the con⁃trol group,the levels of the inflammatory factors IL-4,IL-7,IL-10,IL-17 and IL-33 in the peripheral blood of the obese asthma group were significantly different.Peripheral blood 25(OH)D level in the obese asthma group was lower than that in non-obese asthma group and control group[(18.6±3.2)μg/L vs.(23.1±3.5)μg/L vs.(35.6±6.5)μg/L.P<0.05].Forced expiratory volume in 1 second(FEV1),forced vital capacity(FVC),rate in one second,peak expiratory flow(PEF)and residual capacity/vital capacity(RV/TLV)among the three groups showed statistically significant difference;of the obese asthma group the FEV_(1),rate in one second were lower than those of the non-obese asthma group and the control group,the RV/TLV was higher than those of the non-obese asthma group and the control group,and the FVC and PEF were lower than those of the control group(all P<0.05).There was a statistically significant difference in the frequency distribution of AA,AG,GG genotypes and A and G genotypes of vitamin D receptor genotype rs2228570 in three
作者
张欢欢
王娟
李超
ZHANG Huanhuan;WANG Juan;LI Chao(Department of Neonatal Severe Diseases,Zhumadian First People's Hospital,Zhumadian,Henan 463000,China;Department of Medical Section,Fourth People's Hospital of Zhumadian City,Zhumadian,Henan 463000,China)
出处
《安徽医药》
CAS
2022年第3期564-568,共5页
Anhui Medical and Pharmaceutical Journal
