摘要
研究旨在明确咯菌腈和戊唑醇复配剂抑制小麦赤霉病菌菌丝生长的最佳药剂配比。采用菌丝生长速率法测定了咯菌腈、戊唑醇及其复配剂对小麦赤霉病菌的有效抑制中浓度(EC_(50))。采用wadley的增效比率法,评价了复配剂的增效作用。结果显示,咯菌腈和戊唑醇对小麦赤霉病菌的EC_(50)值分别为0.0205μg/mL和0.1704μg/mL;复配剂咯菌腈:戊唑醇的质量比在40:1,20:1,10:1,5:1,1:1,1:5,1:40时,SR值为0.54~1.28,呈相加作用;复配剂的质量比在1:10,1:20时,SR分别为1.56和1.62,呈增效作用。咯菌腈:戊唑醇的质量比在1:20时,SR值最大,为最佳增效作用比例。本试验为小麦赤霉病的防治及延缓戊唑醇的抗性产生提供了理论依据。
The purpose of this study is to determine the highest synergy ratio(SR)of the mixture of fungicide fludioxonil and tebuconazole that inhibited the mycelial growth of Fusarium graminearum.The effective concentration of 50%(EC_(50))of fludioxonil,tebuconazole and their mixtures against F.graminearum isolates were determined by mycelium growth rate method.The SR of the mixture of the fungicide fludioxonil and tebuconazole was determined by Wadley’s SR method.The synergistic ratio of the mixture of the fungicide fludioxonil and tebuconazole was evaluated.The results showed that the EC_(50) value of fludioxonil and tebuconazole against F.graminearum was 0.0205μg/mL and 0.1704μg/mL,respectively.When the mass ratio of the mixture of fludioxonil and tebuconazole was 40:1,20:1,10:1,5:1,1:1,1:5 and 1:40,the SR range was from 0.54 to 1.28,which showed additive inhibition.When the mass ratio of the mixture of fludioxonil and tebuconazole was 1:10 and 1:20,the SR was 1.56 and 1.62,respectively,which showed synergistic inhibition.When the mass ratio of the mixture of fludioxonil and tebuconazole was 1:20,the SR value was the highest,which was the best synergistic ratio.These results could provide a theoretical basis for the control of wheat scab and delaying the development of tebuconazole resistance.
作者
姜佳
陈金鹏
魏江桥
郭旭昊
车志平
田月娥
陈根强
刘圣明
JIANG Jia;CHEN Jinpeng;WEI Jiangqiao;GUO Xuhao;CHE Zhiping;TIAN Yue’e;CHEN Genqiang;LIU Shengming(Department of Plant Protection,College of Horticultural and Plant Protection,Henan University of Science and Technology,Luoyang,Henan 471023)
出处
《中国农学通报》
2022年第6期116-120,共5页
Chinese Agricultural Science Bulletin
基金
河南省杰出青年科学基金“杀菌剂对禾谷镰孢菌DON毒素合成的影响及其调控机制研究”(212300410015)
中原青年拔尖人才“抗药性禾谷镰孢菌生物学功能研究”(ZYQR201912157)
河南省重大科技专项“小麦条锈病等重大病虫害绿色防控技术研究与应用”(201300111600)
河南省高校科技创新人才支持计划“禾谷镰孢菌DON合成调控机制研究”(20HASTIT033)
河南省高校青年骨干教师培养计划“禾谷镰孢菌抗多菌灵β2-微管蛋白基因与DON毒素合成关系研究”(2018GGJS051)。
关键词
咯菌腈
戊唑醇
小麦赤霉病
禾谷镰孢菌
增效作用
fludioxonil
tebuconazole
wheat scab
Fusarium graminearum
synergistic effect
