摘要
为研究对节白蜡(Fraxinus hupehensis)与“京翠”在分子水平的遗传距离和遗传一致性。利用叶绿体微卫星(Chloroplast microsatellite,cpSSR)分子标记技术,对京山地区采集到的对节白蜡和“京翠”进行亲缘关系研究,并与其他物种共同构建UPGMA聚类图。结果发现,22对cpSSR引物共扩增出了88条多态性带,根据遗传一致度构建2个样本和其他物种数据的UPGMA聚类图,10个样本聚成5个大类:第Ⅰ类包括2个样本,分别为“京翠”和豆科物种;第Ⅱ类为对节白蜡、茄科、猕猴桃科、蔷薇科和桃金娘科;龙舌兰科、禾本科和十字花科分别归属于第Ⅲ、Ⅳ和Ⅴ类。这表明对节白蜡与“京翠”之间存在一定的亲缘性,但是两者亲缘性不高。
In order to study the genetic distance and genetic consistency between Fraxinus hupehensis and“Jingcui”at molecular level.The cpSSR(Chloroplast microsatellite)molecular markers technology were applied to determine the genetic relationship between Fraxinus hupehensis and“Jingcui”collected in Jingshan area,then UPGMA clustering map was constructed through combining the da⁃ta from other species.The results showed that 88 polymorphic bands were amplified by 22 pairs of cpSSR primers,and UPGMA clus⁃tering was constructed for 2 samples according to genetic consistency.The 10 species were clustered into 5 categories,the first catego⁃ry included 2 samples,namely“Jingcui”and Leguminous;the second category was composed of Fraxinus hupehensis,Solanaceae,Actinidiaceae,Rosaceae and Myrtaceae;Agaveaceae,Gramineae and Cruciferae were belong to the III,IV,and V category,sepa⁃rately.The results showed that there was a certain affinity between Fraxinus hupehensis and“Jingcui”,but the affinity was not high.
作者
金宇晨
柴隆龙
马倩
熊燕飞
张建坤
陈碧峰
JIN Yu-chen;CHAI Long-long;MA Qian;XIONG Yan-fei;ZHANG Jian-kun;CHEN Bi-feng(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430014,China;Hubei Senbao Horticulture Co.,Ltd.,Jingmen 431815,Hubei,China)
出处
《湖北农业科学》
2022年第4期160-163,共4页
Hubei Agricultural Sciences
基金
中央高校基本科研业务费专项基金(2020IB029)。
