摘要
目的初步探究鲍曼不动杆菌对不动杆菌属细菌的生长抑制现象。方法2020年12月10日自河南省省立医院神经内科1例危重患者的肺泡灌洗液标本中分离到1株可抑制其他鲍曼不动杆菌生长的鲍曼不动杆菌分离株,命名为SL-A.baumannii。为进一步探究该抑制现象,收集40株不动杆菌分离株,比较抑制作用的发生率、分离株的同源性关系、药敏特征、蛋白质聚类分析等。结果对SL-A.baumannii进行多位点序列分型(MLST),等位基因gltA、gyrB、gdhB、recA、cpn60、gpi和rpoD编号为33、12、40、26、48、54和5,将其原始数据上传到PubMLST数据库后,获得了新的型别号ST-2442。该新型别鲍曼不动杆菌分离株SL-A.baumannii对36株不动杆菌(其中鲍曼不动杆菌30株、皮特不动杆菌3株、洛菲不动杆菌2株、沃森不动杆菌1株)的生长有明显的抑制作用,抑制率达90%(36/40);受其抑制的这36株分离株中全敏感型18株(50%)、多重耐药菌16株(44.44%,含全耐药菌2株、泛耐药菌11株)、其他耐药型2株(5.56%)。结论SL-A.baumannii(PubMLST/鲍曼不动杆菌分离株数据库/编号:6942)这一新型别(ST-2442)的鲍曼不动杆菌野生分离株对不同耐药表型的不动杆菌属细菌生长有显著的抑制作用。
Objectives To investigate the antibacterial effects of Acinetobacter baumannii on Acinetobacter SPP.Methods A Acinetobacter baumannii strain named SL-A.baumannii that can inhibit other Acinetobacter baumannii strains was isolated from the bronchoalveolar lavage fluid of a critically infected patient in the department of neurology,Henan Provincial Hospital on December 10,2020.To better understand this inhibition effect,the drug resistance,homology and protein fingerprint of the new Acinetobacter baumannii strain,along with 40 other Acinetobacter SPP isolates,were analyzed.Results Multilocus sequence typing(MLST)revealed that a novel allele combination(gltA/gyrB/gdhB/recA/cpn60/gpi/rpoD)33,12,40,26,48,54,5 was found in SL-A.baumannii,and ST-2442 was assigned by PubMLST(Oxford).Moreover,36 isolates,including 30 Acinetobacter baumannii strains,2 Acinetobacter lwoffii strains,3 Acinetobacter pittii strains and 1 Acinetobacter ursingii strain,were inhibited by SL-A.baumannii,the inhibition rate was 90%(36/40).Among those strains,18(50%)were sensitive to all the antibacterial drugs;16(44.44%)showed multi-drug resistance(MDR),including 2 pan-drug resistance(PDR)and 11 extensive drug resistance(XDR)strains;the other 2(5.56%)were only resistant to Trimethoprim/Sulfamethoxazole(SXT).Conclusion The new sequence type(ST-2442)Acinetobacter baumannii isolate SL-A.baumannii(PubMLST/Acinetobacter baumannii isolates database/id:6942)had shown a significantinhibitory effect on Acinetobacter SPP with different drug-resistant phenotypes.
作者
樊路娟
酒康楠
刘智超
Fan Lujuan;Jiu Kangnan;Liu Zhichao(Department of Clinical Laboratory,the First affiliated Hospital of Zhengzhou University,Key Clinical Laboratory of Henan Province,Zhengzhou 450052,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2022年第2期169-173,共5页
Chinese Journal of Laboratory Medicine
