摘要
为深入了解不同生长年限桔梗根部基因表达差异,本研究以1年及3年生紫花桔梗根部为试验材料进行转录组分析。共获取41.84 G clean base,拼接后获得54 425个Unigene,平均长度为1 352 bp。筛选得到3 700个DEGs,其中2 514个在3年生样本中上调表达,1 186个下调表达。3 700个差异表达基因共富集到2 975个GO项,52项显著富集。52项显著富集中,分子功能28项,生物学过程21项,细胞组分3项。KEGG富集表明,上调DEGs显著富集到植物病原体互作、淀粉蔗糖代谢、苯丙烷类生物合成和植物激素信号传导途径,下调DEGs显著富集到光合作用-天线蛋白、光合作用、玉米素生物合成途径。同时对各个途径中显著差异表达基因注释到的差异相关蛋白、转录因子及关键酶基因进行挖掘。荧光定量PCR的表达与转录组测序结果的一致性说明转录组测序数据可靠。该研究通过分析不同生长年限桔梗根部转录水平上的差异,有助于理解不同生长年限桔梗产量、结实率及植株抗病性等方面存在的差异,为后期开展桔梗分子育种、生长发育及相关功能基因解析提供基础信息。
To understand the gen e expression differences in the roots of purple flower P. grandiflorum with different growth years, transcriptome analysis was carried out in 1-year-old and 3-year-old P. grandiflorum roots.A total of 41.84 G clean bases were obtained. 54 425 Unigenes were obtained after splicing, with an average length of 1 352 bp. Among 3 700 DEGs, 2 514 were up-regulated and 1 186 were down regulated. 3 700 DEGs were annotated in 2 975 GO items, 52 of them were significantly enriched. 52 items were significantly enriched including28 groups in molecular function, 21 in biological process, and 3 in cell components. KEGG enrichment indicated that up-regulated DEGs were significantly enriched in plant-pathogen interaction, starch sucrose metabolism,phenylpropanoid biosynthesis and plant hormone signal transduction pathways, while down regulated DEGs were significantly enriched in photosynthesis-antenna proteins, photosynthesis and Zeatin biosynthesis pathways.Meanwhile, the differentially expressed proteins, transcription factors and key enzyme genes annotated by DEGs in each pathway were explored. Consistency between expression of RT-qPCR and transcriptome sequencing results indicated that transcriptome sequencing data are reliable. By analyzing differences in gene transcription levels of P.grandiflorum roots with different growth years, it is helpful to understand the differences in yield, seeds setting rate and plant disease resistance of P. grandiflorum in different growth years. This study provides basic information for molecular breeding, detecting plant growth and developing related functional gene of P. grandiflorum.
作者
宋旭红
崔广林
丁刚
李隆云
Song Xuhong;Cui Guanglin;Ding Gang;Li Longyun(Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Academy of Chinese Materia Medica,Chongqing,400065)
出处
《分子植物育种》
CAS
北大核心
2022年第2期396-407,共12页
Molecular Plant Breeding
基金
国家中药材产业技术体系项目(CARS-21)
重庆市现代山地特色高效农业技术体系项目(2020[9]号)共同资助。
