摘要
目的用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术调查采供血机构环境污染情况,评价应用价值。方法采集消毒后献血场所空气、献血者皮肤穿刺部位、血小板保存箱、血小板采集设备、相关实验耗材的物体表面和工作人员袖口表面,培养后挑选典型菌落使用微生物质谱技术进行鉴定,与2017~2021年5月间血液成分中检出细菌鉴定结果比对。结果无菌生长占21.20%,菌落数≤4.0 CFU/皿占62.20%,菌落数>4.0 CFU/皿占16.60%,合格率从高到低依次为血小板保存箱、消毒后献血场所空气中沉降菌、血小板采集设备、消毒后献血者皮肤穿刺部位、血小板耗材表面、医护人员工作服表面,消毒后献血者皮肤穿刺部位与其他采集位置检测菌落数进行比较,t值分别为2.0371、1.508、2.109、1.961和1.778,均无显著性差异(P>0.05);回顾分析2017~2021年5月血液成分细菌污染,检出30例细菌,其中单采血小板被检出率最高,单采血小板与红细胞类和血浆细菌污染次数比较,t值分别为1.731和2.272,均无显著性差异(P>0.05),与浓缩血小板污染次数比较,t值为2.875,具有显著性差异(P<0.05)。结论血液成分污染细菌多来源于空气菌群、献血者手臂皮肤消毒后残留菌群、相关设备物料表面,严格做好采供血场所的消毒工作,建立消毒监测方法、制定血站行业的消毒卫生标准,对降低细菌污染有临床意义。
Objective To investigate the environmental pollution of blood collection and supply institutions by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) and evaluate its application value.Methods Colonies of air from blood donation sites, skin puncture sites of blood donors, platelet storage boxes, platelet collection equipment, object surfaces of related experimental consumables and cuff surfaces of staff after disinfection were collected, and typical colonies after cultivation were selected for microbial identification by microbial mass spectrometry and then compared with bacteria results detected in blood components from May 2017 to May 2021. Results Aseptic growth, the number of colonies ≤4.0 CFU/dish, and the number of colonies > 4.0 CFU/dish accounted for 21.20%, 62.20% and 16.60%, respectively. The qualified rate from high to low was platelet storage box, bacteria settling in the air of blood donation room after disinfection, platelet collection equipment, skin puncture site of blood donors after disinfection, the surface of platelet consumables and the surface of medical staff’s overalls. After disinfection, the blood donors’ skin puncture sites were compared with other collection sites, and the t values were 2.0371, 1.508, 2.109, 1.961 and 1.778, respectively, with no significant difference(P>0.05). Thirty cases of bacterial contamination of blood components were detected from May 2017 to May 2021, among which the detection rate of apheresis platelets was the highest, and the t values were 1.731 and 2.272, relative to the contamination frequency of erythrocytes and plasma bacteria(P>0.05), while the t value was 2.875, relative to concentrated platelets, with significant difference(P<0.05). Conclusion Bacterial contamination of blood components mostly come from air bacteria settling, blood donors’ arms and skin after disinfection, and surfaces of related equipment and materials. Therefore, it is of clinical significance to conduct strict disinfection of
作者
章舜玮
周燕
胡天彦
陈英姣
杨剑豪
吴佳琪
邱颖婕
ZHANG Shunwei;ZHOU Yan;HU Tianyan;CHEN Yingjiao;YANG Jianhao;WU Jiaqi;QIU Yingjie(Shanghai Blood Grnter,Shanghai 100051 China)
出处
《中国输血杂志》
CAS
2022年第2期209-212,共4页
Chinese Journal of Blood Transfusion
关键词
基质辅助激光解吸电离飞行时间质谱
采供血环境
微生物鉴定
matrix-assisted laser desorption ionization time-of-flight mass spectrometry
environment of blood collection and supply institutions
microbial identification
