摘要
目的:建立一种简单的高效液相色谱法用于测定人血清中替加环素的浓度。方法:以盐酸米诺环素为内标,乙腈沉淀蛋白法处理样品。色谱柱为KromasilC18柱(4.6mm×150mm,5μm);以乙腈-磷酸盐缓冲液(24∶76,v/v,pH=3.0)为流动相,流速1mL·min^(-1);检测波长246nm;进样量50μL。结果:人血清中替加环素的校准曲线在0.10~5.00μg∙mL^(-1)浓度范围内线性关系良好。低、中、高质量浓度质控样品的日内和日间精密度RSD均<10%,提取回收率在99.43%~116.68%。结论:该方法简便、可靠、灵敏度高,已成功应用于我院接受替加环素治疗患者的样本分析。
Objective:To establish a simple high performance liquid chromatography(HPLC)method for the determination of tigecycline(TGC)in human serum.Methods:The samples were treated by acetonitrile precipitation protein method with minocycline hydrochloride as internal standard.The chromatographic column was Kromasil C18 column(4.6 mm×150 mm,5μm).The mobile phase consisted of acetonitrile phosphate buffer(24∶76,v/v,pH=3.0).The flow rate was 1 mL∙min^(-1) and detection wavelength was 246 nm.Injection volume was 50μL.Results:The calibration curve of TGC in human serum has a good linearity in the concentration range of 0.10–5.00μg∙mL^(-1).The intraday and interday precision RSD of low,medium,high-dose quality control samples were<10%respectively.Extraction recovery was 99.43%–116.68%.Conclusion:This method was simple and reliable with high sensitivity,which was successfully applied for the determination of TGC in patients of our hospital.
作者
李文超
吴燕川
沈芊
苏甦
李晓玲
白向荣
LI Wen-chao;WU Yan-chuan;SHEN Qian;SU Su;LI Xiao-ling;BAI Xiang-rong(Department of Pharmacy,Xuanwu Hospital,Capital Medical University/National Clinical Research Center for Geriatric Disorders,Beijing 100053,China;School of Pharmacy,Capital Medical University,Beijing 100053,China;Central Laboratory of Xuanwu Hospital,Capital Medical University,Beijing 100053,China)
出处
《中国药物应用与监测》
CAS
2022年第1期16-20,共5页
Chinese Journal of Drug Application and Monitoring
基金
北京市科学技术委员会“老年人多重用药管理模式的建立与临床应用研究”专项资助课题(D181100000218002)
北京市卫生和计划生育委员会“老年重大疾病关键技术研究”项目(PXM2018_026283_000002)
北京市属医院科研培育项目(PX2020038)。
关键词
替加环素
血药浓度
高效液相色谱法
医院获得性肺炎
治疗药物监测
Tigecycline
Blood drug concentration
High performance liquid chromatography
Hospital-acquired pneumonia
Therapeutic drug monitoring
