摘要
目的探究肝癌患者肿瘤教育血小板源性微小RNA(miRNA)-122和miRNA-21的表达水平及其对肝癌的诊断价值。方法选取2021年12月在该院诊治的15例原发性肝癌患者及同期在该院行健康体检的15例体检健康者分别作为肝癌组和健康对照组。采用低速离心法提取受试者血浆中的血小板,采用实时荧光定量PCR检测富血小板悬液中教育血小板源性miRNA-122和miRNA-21的相对表达量,并采用受试者工作(ROC)曲线分析它们在肝癌诊断方面的临床价值。结果肝癌组教育血小板源性miRNA-122和miRNA-21的相对表达量均上调,差异均有统计学意义(P<0.05)。miRNA-122和miRNA-21诊断原发性肝癌的曲线下面积(AUC)分别为0.987和0.916。结论肝癌组血浆中教育血小板源性miRNA-122和miRNA-21的表达水平明显升高,其相对表达量可以有效区分肝癌组和健康对照组,肿瘤教育血小板源性miRNA-122和miRNA-21有望成为诊断肝癌的潜在分子标志物。
Objective To detect the expression levels of miRNA-532 and miRNA-221 in tumor-educated platelets(TEP)of liver cancer patients,and to explore their diagnostic value for liver cancer.Methods Fifteen patients with liver cancer treated in the hospital in December 2021 and 15 healthy people who underwent physical examination in the hospital in the same period were selected as liver cancer group and healthy control group.Low-speed centrifugation was used to extract the platelets from the subjects′plasma,and real-time fluorescent quantitative PCR was used to detect the relative expression of TEP miRNA-122 and miRNA-21.The receiver operating characteristic(ROC)curve was used to analyze their clinical value in the diagnosis of liver cancer.Results The relative expression levels of TEP miRNA-122 and miRNA-21 in liver cancer patients group level higher than those in the control group,the differences were statistically significant(P<0.05).The area under the curve(AUC)of TEP miRNA-122 and miRNA-21 for diagnosing primary liver cancer were 0.987 and 0.916,respectively.Conclusion The expression levels of TEP miRNA-122 and miRNA-21 in liver cancer patients′plasma are significantly increased,and their relative expression levels can effectively distinguish liver cancer patients from healthy controls.TEP miRNA-122 and miRNA-21 are expected to be potential molecular markers for the diagnosis of liver cancer.
作者
余恋雨
王宇
唱凯
陈鸣
YU Lianyu;WANG Yu;CHANG Kai;CHEN Ming(Department of Clinical Laboratory,the First Affiliated Hospital of Army Medical University,Chongqing 400038,China)
出处
《国际检验医学杂志》
CAS
2022年第5期519-522,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金面上项目(81972027)。
