摘要
目的分析艰难梭菌基因组中耐药基因、毒力基因和核心基因的分布,明确该菌耐药和毒力的分子特征。方法采用Prokka和FastTree等软件分析已公布的艰难梭菌2455个基因组的核心基因和系统发生树,并进一步采用Abricate软件和自主开发的软件解析不同核心基因组和分子分型对应的耐药基因和毒力基因的分布特征,明确耐药基因、毒力基因与基因组型的关系。结果基于基因组学特征,CladeⅣ为新发现的进化分支,Clade C-Ⅰ/Ⅱ/Ⅲ发现了新的分子分型,万古霉素耐药性的发生具有进化聚集性,临床高毒菌株的基因组分布日益广泛。核心基因组比较分析表明Clade 5中细胞运动性相关基因占比最小。结论通过分析已发表的艰难梭菌基因组序列,描绘该菌耐药基因、毒力基因的进化聚集性、广泛性和核心基因的功能特异性,有助于对该菌的院内传播和流行病学进行研究,为改进临床治疗策略提供更多依据。
Objective To investigate the distribution of antibiotic resistance genes,virulence genes and core genes in Clostridium difficile(C.difficile)genomes,and to identify the molecular characteristics of antimicrobial resistance and virulence of C.difficile.Methods The core genes and phylogenetic tree of 2455 C.difficile genomes were analyzed by Prokka and FastTree software.Abricate software and self-developed software were furtherly used to analyze the distribution characteristics of antibiotic resistance genes and virulence genes corresponding to different core genomes and molecular types,so as to clarify the relationship between drug resistance genes,virulence genes and genotype.Results Based on genomic characteristics,CladeⅣis a newly discovered evolutionary branch.New molecular typings were found in Clade C-Ⅰ/Ⅱ/Ⅲ,and the occurrence of vancomycin resistance showed evolutionary aggregation.The genomes of clinical strains with high virulence were widely distributed.The comparative analysis of the core genomes showed that the proportion of cell motility related genes in Clade 5 was the smallest.Conclusions By analyzing the published genome sequences of C.difficile,the distributive characteristics of antimicrobial resistance genes,virulence genes and core genes were described,which could provide more basis for better understanding of the bacterium and improvement of clinical treatment strategies.
作者
岳靖林
滑明溪
段昂
杜鹏程
霍成
陈晨
Yue Jinglin;Hua Mingxi;Duan Ang;Du Pengcheng;Huo Cheng;Chen Chen(Institute of Infectious Diseases,Peking University Ditan Teaching Hospital,Beijing 100015,China;Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China;Pathology Department,Sinopharm Tongmei General Hosptial,Datong 037001,China)
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2021年第6期409-418,共10页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
国家重点研发计划(No.2018YFE0192500)。
关键词
艰难梭菌
耐药基因
毒力基因
核心基因
Clostridium difficile
Antimicrobial resistance gene
Virulence gene
Core gene
