摘要
以OSMAC策略为指导,研究深海真菌Stachybotrys sp.3A00409的次级代谢产物多样性,对其燕麦大米培养基发酵产物中与原始培养基具有差异的部分进行靶向分离,共得到7个单体化合物,鉴定为丁内酯类化合物butyrolactone-Ⅰ(1)、butyrolactone-Ⅲ(2)、butyrolactone-Ⅱ(3)、2-O-methylbutyrolactone-Ⅱ(4)、萘类化合物methyl-6-acetyl-4-methoxy-5,7,8-trihydroxy-naphthalene-2-carboxylate(5)、吡喃酮衍生物pyrophen(6)和阿魏酸(7)。抗肿瘤实验结果显示化合物1,2,7对人口腔上皮癌KB细胞表现出了较强的增值抑制作用,IC_(50)在1.18~1.34μg∙mL^(-1)。
Under the guidance of OSMAC strategy,the diversity of secondary metabolites from deep-sea fungus Stachybotrys sp.3A00409 was explored,and the chemical composition and biological activity of fermentation products from rice and oat medium were studied.Seven compounds were isolated from the fermentation extract of rice and oat medium of Stachybotrys sp.3A00409,which were identified as butyrolactone-Ⅰ(1),butyrolactone-Ⅲ(2),butyrolactone-Ⅱ(3),2-O-methylbutyrolactone-Ⅱ(4),methyl-6-acetyl-4-methoxy-5,7,8-trihydroxy-naphthalene-2-carboxylate(5),pyrophen(6)and ferulic acid(7).Compound 1,2,7 showed proliferation inhibition against human oral epithelial carcinoma KB cells with IC_(50) values raging from 1.18 to 1.34μg∙mL^(-1).
作者
彭艾
左满
王乾军
张帆
胡佳
胡茂军
沈锦煌
钟添华
骆祝华
马新华
Peng Ai;Zuo Man;Wang Qianjun;Zhang Fan;Hu Jia;Hu Maojun;Shen Jinhuang;Zhong Tianhua;Luo Zhuhua;Ma Xinhua(School of Pharmacy,Fujian Medical University,Fuzhou 350122,China;Key Laboratory of Biogenetics Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361006,China)
出处
《山东化工》
CAS
2022年第3期19-21,24,共4页
Shandong Chemical Industry
基金
国家自然科学基金项目(41806173)
福建医科大学“大学生创新创业训练计划”项目(C20152)。
