摘要
背景:九仙罗汉接骨汤具有活血散瘀、接骨消肿、止痛的作用,但目前其对胫骨骨折的具体作用机制尚未完全掌握。目的:探究苗药九仙罗汉接骨汤介导BMPs/Smad信号通路对胫骨骨折大鼠成骨分化及骨折愈合能力的影响。方法:60只SD大鼠随机分为模型组、苗药组、三七组,采用改良钻孔法建立不完全性胫骨骨折模型。造模成功当天,苗药组给予苗药九仙罗汉接骨汤煎剂(2 mL/kg)灌胃;三七组给予同体积三七粉液(1 mL/kg)灌胃;模型组给予同等计量蒸馏水灌胃,1次/d,共28 d。28 d后麻醉下取大鼠胫骨组织,茜素红染色测骨髓间充质干细胞成骨分化;三点弯曲试验测胫骨生物力学指标;苏木精-伊红染色测骨组织形态;ELISA检测血清中血管内皮生长因子、胰岛素样生长因子1表达水平;RT-PCR和Western blot检测骨形态发生蛋白2、转化生长因子β、Smad1、Smad2的mRNA和蛋白水平;实验已经过贵州中医药大学第二附属医院动物伦理委员会批准(2018-072)。结果与结论:①与模型组比较,苗药组与三七组骨髓间充质干细胞成骨分化后细胞多层重叠生长且形成灶状,有大量的钙结节形成,且苗药组较三七组效果更佳;②与模型组相比,三七组与苗药组骨形态发生蛋白2、转化生长因子β、Smad1、Smad2的mRNA和蛋白水平均有所上升(P<0.05);③三七组与苗药组最大载荷、刚度水平较模型组均有所升高(P<0.05);④模型组骨小梁较细,髓腔间空隙大,杂乱无章,较为松散,有大量的未钙化软骨细胞;三七组与苗药组大鼠骨组织形态较为接近,均表现为骨小梁较粗,髓腔空隙减小,分布较为有序,软骨细胞骨化较多;⑤与模型组相比,三七组与苗药组血管内皮生长因子、胰岛素样生长因子1表达水平有一定程度的提升(P<0.05);⑥模型组大鼠存在明显骨折线,三七组与苗药组标本骨折断端均达到完全愈合,但与三七组相比,苗药组标本骨�
BACKGROUND:Jiuxian Luohon Jiegu Decoction can promote blood circulation,remove blood stasis,reduce swelling,and relieve pain.However,its specific mechanism underlying tibial fractures has not yet been fully elucidated.OBJECTIVE:To explore the effect of Miao medicine Jiuxian Luohan Jiegu Decoction on the osteogenic differentiation and fracture healing ability of rats with tibial fractures mediated by bone morphogenetic proteins(BMPs)/Smad signaling pathway.METHODS:Sixty Sprague-Dawley rats were randomly divided into a fracture group,a Miao medicine group,and a Panax notoginseng group.The Miao medicine group was given Jiuxian Luohon Jiegu Decoction(2 mL/kg)by gavage;the Panax notoginseng group was given the same volume of panax notoginseng powder(1 mL/kg)by gavage;and the model group was given the same amount of distilled water by gavage,once a day,for 28 days in total.Alizarin red staining was used to measure osteogenic differentiation of bone marrow mesenchymal stem cells.RT-PCR and western blot were used to measure BMP-2,transforming growth factor-β,Smadl,and Smad2 at mRNA and protein levels.Three-point bending test was used to measure biomechanical indexes.Hematoxylin-eosin staining was used to detect bone tissue morphology.ELISA was used to measure serum vascular endothelial growth factor and insulin growth factor-1.RESULTS AND CONCLUSION:(1)Compared with the model group,the differe ntiated cells of the Miao medicine group and the Panax notoginseng group grew in multiple layers and fo rmed focal shapes,and a large number of calcium nodules were formed.The Miao medicine group showed better effects than the Panax notoginseng group.(2)Compared with the model group,the mRNA levels of BMP-2,transforming growth factor-β,Smad1,and Smad2 were increased in the Panax notoginseng group and the Miao medicine group increased(P<0.05).(3)The maximum load and stiffness of the panax notoginseng group and the Miao medicine group were higher than those of the model group(P<0.05).(4)The bone trabeculae of the model group were t
作者
李义
杨彦君
彭松云
程志刚
钟凯
殷天坪
唐良华
Li Yi;Yang Yanjun;Peng Songyun;Cheng Zhigang;Zhong Kai;Yin Tianping;Tang Lianghua(Second Department of Orthopedics,the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,Guizhou Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第33期5350-5356,共7页
Chinese Journal of Tissue Engineering Research
基金
贵州省科学技术厅科技核心计划项目(黔科合LH字[2015]7257号),项目负责人:唐良华。
关键词
苗药九仙罗汉接骨汤
BMPs/Smad信号通路
成骨分化
骨折愈合
Miao Medicine Jiuxian Luohan Jiegu Decoction
BMPs/Smad signaling pathway
osteogenic differentiation
fracture healing
