摘要
微流控技术已成为目前发酵工程中筛选和表征微生物性能的重要方法。本研究从微流控技术的理论出发,将计算流体力学、颗粒-流体两相流动和牛顿运动定律理论用于粒子追踪模型,建立了毕赤酵母微胶囊收集过程的数学模型。借助仿真软件模拟了毕赤酵母微胶囊的收集过程,设计了微流控芯片中收集器结构,并进行了进一步的数值模型模拟,最后搭建了用于分析毕赤酵母微胶囊的实验平台。本研究为研究毕赤酵母单细胞表达重组蛋白奠定了一定基础。
Microfluidics has become an important method for screening and characterizing the performance of microorganisms in fermentation engineering.In this project,a mathematical model of the microcapsule collection process was developed according to the theory of microfluidics,particle-fluid two-phase flow and Newton’s laws of motion solid mechanics,using particle tracking models.The collection process of Pichia pastoris microcapsules was simulated with the help of simulation software,the collector structure in the microfluidic chip was designed,and further numerical model simulations were carried out.Then,the collector was designed and set up for experimental verification of microcapsule collection and analysis.This research lays a foundation for research about recombinant protein expression by recombinant K.phaffii.
作者
周紫薇
项郑昊
周化岚
张建国
ZHOU Zi-wei;XIANG Zheng-hao;ZHOU Hua-lan;ZHANG Jian-guo(Institute of Food Science and Engineering,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《食品与发酵科技》
CAS
2022年第1期67-71,共5页
Food and Fermentation Science & Technology
基金
国家自然科学基金面上项目(31870045)。
关键词
微流控芯片
毕赤酵母
微胶囊
重组蛋白表达
microfluidics
Komagataella phaffii
microcapsules
recombinant protein expression
