摘要
旨在研究鸽毛滴虫与白色念珠菌二联卵黄抗体制备方法和评价其临床效果。利用临床分离的白色念珠菌与毛滴虫制备二联灭活疫苗,免疫产蛋母鸡获得高水平抗体,采取低温冻干技术制备成卵黄抗体粉。建立ELISA抗体检测法测定卵黄抗体水平,确保质量可控;以分离的肉鸽毛滴虫、白色念珠菌制备发病模型,评价卵黄抗体的保护效果。结果表明:二次免疫后,卵黄中毛滴虫抗体和白色念珠菌抗体OD值达到高峰,分别为0.90和0.66,抗体维持时间达到2个月。卵黄抗体按1 g·只^(-1)口服饲喂5 d后,肉鸽口腔和嗉囊病变评分与感染对照组相比显著降低(P<0.05),与甲硝唑、制霉菌素无差异;此外,毛滴虫、白色念珠菌病原载量极显著降低(P<0.01)。研制的鸽毛滴虫与白色念珠菌二联卵黄抗体可以替代甲硝唑和制霉菌素,为肉鸽的健康养殖提供一种新的抗菌素替代产品。
This study aimed to prepare the dual yolk antibody of Trichomonas gallinae and Candida albicans and study its therapeutic effect.Based on isolates of T.gallinae and C.albicans,we prepared a bivalent vaccine,harvested high-level antibody by inoculating to layers and produced into powders using freeze-dry system under low temperature.Moreover,antibody levels were determined to check quality control using in-house ELISA method.To test efficacy,pigeon models were developed by artificial infection with T.gallinae and C.albicans,respectively.The OD values of T.gallinae antibody and C.albicans antibody in the yolk reached their peaks after second immunization,which were 0.90 and 0.66,respectively,and the antibody maintenance time reached 2 months.In vivo study,both oral-and crop lesions score were reduced significantly(P<0.05)post feeding with 1 g of hyperimmune antibody powders per bird for 5 days and no significant difference was found compared to those pigeons treated with metronidazole and nystatin.Moreover,pathogen loads were reduced greatly compared to the control group(P<0.01).The hyperimmune antibody powder is a promising approach to replace metronidazole and nystatin,contributing to a novel solution for healthy development of pigeon industry.
作者
王飞
张禾
蔡淯涵
黄中佐
严沛洁
王李格
孙鸿
李复煌
何诚
WANG Fei;ZHANG He;CAI Yuhan;HUANG Zhongzuo;YAN Peijie;WANG Lige;SUN Hong;LI Fuhuang;HE Cheng(College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;Beijing Animal Husbandry Station, Beijing 100107, China;Beijing Youdi Pigeon Industry Co., Ltd., Beijing 100188, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2022年第2期658-662,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
北京市大学生创新创业实践项目(Bj202010019287X)。
关键词
肉鸽
毛滴虫
白色念珠菌
卵黄抗体粉
甲硝唑
制霉菌素
meat pigeon
Trichomonas gallinae
Candida albicans
hyperimmune antibody powder
metronidazole
nystatin
