摘要
建立一种磁分离辅助荧光分析检测砂糖橘中的3种常见有机磷农药的方法。采用溶剂热法合成磁珠,并结合聚吡咯(polypyrrole,PPy)修饰,形成Fe_(3)O_(4)@PPy纳米粒子作为荧光猝灭剂。利用6-羧基荧光素标记适配体S4-29广谱性识别有机磷农药的特性,建立磁分离辅助荧光分析法检测砂糖橘中甲拌磷、氧化乐果、丙溴磷3种有机磷农药的检测方法。在Fe_(3)O_(4)纳米粒子和PPy质量比1∶1.5、Fe_(3)O_(4)@PPy纳米粒子质量浓度0.2 mg/mL、孵育时间120 min条件下,甲拌磷、氧化乐果、丙溴磷的检出限分别为4.17×10^(-4)、2.77×10^(-4)、4.67×10^(-4)μg/kg;回收率为79.94%~108.00%;相对标准偏差为3.05%~7.39%。该方法选择性强、准确度高,为食用农产品中禁用有机磷农药检测提供研究思路。
In this paper,a magnetic separation assisted fluorescence method for the determination of three common organophosphorus pesticides(OPPs)in‘Shatangju’mandarin was established.Magnetic beads were synthesized by solvothermal method and modified by polypyrrole(PPy)to form Fe_(3)O_(4)@PPy nanoparticles as a fluorescence quenching agent.This method was based on the use of a 6-carboxyfluorescein(FAM)-labeled aptamer(S4-29)by virtue of its broadspectrum recognition ability for OPPs.When Fe_(3)O_(4)/PPy nanoparticles mass ratio,Fe_(3)O_(4)@PPy nanoparticles concentration and incubation time were 1:1.5,0.2 mg/mL,and 120 min,respectivley,the detection limits of phorate,omethoate and profenofos were 4.17×10^(-4),2.77×10^(-4),and 4.67×10^(-4)μg/kg,and the recoveries ranged from 79.94%to 108.00%with relative standard deviations(RSDs)between 3.05%and 7.39%.In conclusion,this method has strong selectivity and high accuracy and can be used for the analysis of banned OPPs in agricultural products.
作者
胡超琼
王力均
陶璇
朱云
梁煜
杨潇
陈祥贵
黄玉坤
HU Chaoqiong;WANG Lijun;TAO Xuan;ZHU Yun;LIANG Yu;YANG Xiao;CHEN Xianggui;HUANG Yukun(School of Food and Biological Engineering,Xihua University,Chengdu 610039,China;Key Laboratory of Food Non Thermal Processing,Engineering Technology Research Center of Food Non Thermal Processing,Yibin Xihua University Research Institute,Yibin 644004,China;Chengdu Brilliant Pharmaceutical Co.Ltd.,Chengdu 610041,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第4期264-269,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(31801647)
四川省科技厅应用基础研究项目(2018JY0194,2020YFN0151)
西华大学省部级学科平台开放课题(szjj2017-115)。
关键词
适配体
磁分离
有机磷农药
荧光分析法
砂糖橘
aptamer
nanomagnetic beads
organophosphorus pesticides
fluorescence analysis
‘Shatangju’mandarin
