摘要
目的研究弥漫大B细胞淋巴瘤中p53蛋白的表达及临床病理特征的分析。方法研究对象选取2006⁃07—2017⁃09期间收治并确诊的224例弥漫大B细胞淋巴瘤患者,收集患者临床及病理学资料并采用免疫组织化学方法检测石蜡组织中p53蛋白表达,分析其与临床病理资料及患者预后的相关性,寻找预后相关最佳CUT OFF值。结果肿瘤细胞阳性比≥30%时p53预后相关意义最显著(χ^(2)=8.174,P=0.004),p53蛋白表达阳性率约37.5%(84/224),阳性组PFS(χ^(2)=8.174,P=0.004)及OS(χ^(2)=8.636,P=0.003)显著低于阴性组。多因素分析显示年龄、Hans分型、Ann Arbor临床分期、IPI评分及p53蛋白表达均是DLBCL独立预后因素(χ^(2)=53.951,P<0.001)。p53蛋白表达与临床病理特征年龄、性别、首次发病部位、Hans分型、Ann Arbor临床分期及IPI评分之间无显著相关性(P<0.05)。结论p53蛋白阳性表达是DLBCL独立预后因素,年龄、Hans亚型、Ann Arbor临床分期、IPI评分及p53蛋白表达均可作为DLBCL预后评估指标。
Objective To investigate the expression of p53 protein in diffuse large B⁃cell lymphoma(DLBCL)tissues and to analyze the clinicopathological characteristics of diffuse large B⁃cell lymphoma.Methods A total of 224 cases of DLBCL were selected during July 2006 to September 2017 in our hospital during July 2006 to September 2017.Clinical and pathological data of patients were collected and the expression level of p53 protein was detected by immunohistochemical method.Statistical method was used to analyze relevance between expression level of p53 protein and the prognosis of patients,as well as Clinical and pathological characteristics and to find the optical CUT OFF value related to prognosis.Results The prognostic significance of p53 was the most significant when the positive ratio of tumor cells was≥30%(P=0.004,χ^(2)=8.174).The positive rate of p53 protein expression was about 37.5%(84/224),and the PFS(P=0.004,χ^(2)=8.174)and OS(P=0.003,χ^(2)=8.636)of the positive group were significantly lower than those of the negative group.Multivariate analysis showed that age,Hans classification,Ann Arbor clinical stage,IPI score and p53 protein expression were all independent prognostic factors for DLBCL(P<0.001,χ^(2)=53.951).There was no significant correlation between p53 protein expression and clinicopathological characteristics including age,gender,location of first onset,Hans classification,Ann Arbor clinical stage,and IPI score(P<0.05).Conclusion The positive expression of p53 protein is an independent prognostic factor of DLBCL.Age,Hans classification,Ann Arbor clinical stage,IPI score and p53 protein expression can all be used as prognostic indicators for DLBCL.
作者
李红艳
于国华
隋小龙
姜蕾
王通
曲桂梅
LI Hong-yan;YU Guo-hua;SUI Xiao-long;JIANG Lei;WANG Tong;QU Gui-mei(Department of Pathology,Yuhuangding Hospital Affiliated to Qingdao University,Yɑntɑi 264000,China)
出处
《诊断病理学杂志》
2022年第1期6-11,共6页
Chinese Journal of Diagnostic Pathology
基金
山东省自然科学基金(ZR2017MH081)。
