摘要
【目的】通过研究不同颜色稻米的生理功能效应,分析其具有生理功能效应的活性成分,从而为保健功能稻米品种选育提供理论依据。【方法】采用凯氏定氮法测总蛋白含量,蒽酮比色法测总淀粉含量,Megazyme试剂盒测抗性淀粉含量,福林酚法测总的多酚含量,NaNO_(2)-Al(NO)_(3)比色法测类黄酮含量,ORAC法进行体外抗氧化活性检测,通过昆明小鼠进行体内血糖血脂效应研究。【结果】试验采用的3种不同颜色稻米材料分别是白米(蜀恢527)、红米(红香糯)和黑米(黑香糯)。蛋白、淀粉和抗性淀粉检测结果表明3个不同颜色的水稻品种的总蛋白含量、总的淀粉和抗性淀粉含量无显著差异;总的多酚含量结果表明,红米品种中的多酚含量最高(12.6 mg/g),其次是黑米(8.0 mg/g),白米含量最低(5.6 mg/g);在3个不同颜色水稻品种中的类黄酮含量的变化趋势与多酚一致。体外抗氧化活性结果表明,红米红香糯的氧化自由基吸收能力最高为363μmol Trolox/g,其次是黑米黑香糯为219μmol Trolox/g,最低的是白米蜀恢527,仅107μmol Trolox/g;体内降糖和降脂活性研究表明,红米红香糯提取物表现出降糖趋势,但是降脂效应确在黑米黑香糯组呈现;更进一步的体内SOD活性研究表明,红米红香糯组小鼠表现最强活性,达到556.65 U/mg。【结论】红米红香糯和黑米黑香糯富含丰富的多酚和类黄酮,白米蜀恢527含量最低,因此红米和黑米具有强的生理功能,尤其是红米红香糯。
【Objective】The study compared physiological function in different pigmented rice varieties and analyzed activity components,in order to provide theoretical basis for breeding of rice.【Method】The study measured the total protein content using the Kjeldahl method,the total starch content by colorimetric method,the content of resistant starch by the Megazyme kit.The total polyphenol was checked using the Folin method.The flavonoid content was measured by the NaNO_(2)-Al(NO)_(3) colorimetric method.The study also detected the antioxidant activity by ORAC method in vitro,and blood sugar and lipid by Kunming mice experiment.【Result】Three different pigmented rice varieties were used to determine the total protein content,starch content,total phenolic and flavonoid content.Three rice materials were as follows:white rice(Shuhui 527),red rice(Hongxiangnuo)and black rice(Heixiangnuo).The results showed that the total protein,starch and resistant starch content hardly existed in significant differences among three different pigmented rice varieties.The results of total polyphenols content showed that red rice Hongxiangnuo(12.6 mg/g)was the highest,following by black rice Heixiangnuo(8.0 mg/g),the lowest was white rice Shuhui527(5.6 mg/g).Besides,the tendency of total flavonoid content in three rice varieties was the same as that of polyphenol content.The results of antioxidant capacity showed that red rice Hongxiangnuo(363μmol Trolox/g)for antioxidant activity presented the highest,following by black rice Heixiangnuo(219μmol Trolox/g),the lowest was white rice Shuhui 527(107μmol Trolox/g)by vitro.Red rice Hongxiangnuo was stronger for hypoglycemic by vivo.On the contrary,blood lipids level reduced just presented in black rice Heixiangnuo group.The study further found that red rice Hongxiangnuo group had the highest SOD value as the same as antioxidant activity in vitro,and attained 556.65 U/mg.【Conclusion】Red rice(Hongxiangnuo)especially possesses the strongest physiological activity,following by black ric
作者
郭巧玲
杨莹
杨小莉
林郸
强润润
加峰
张红宇
徐培洲
廖泳祥
王静
周浩
刘禹彤
吴先军
陈晓琼
GUO Qiaoling;YANG Ying;YANG Xiaoli;LIN Dan;QIANG Runrun;JIA Feng;ZHANG Hongyu;XU Peizhou;LIAO Yongxiang;WANG Jing;ZHOU Hao;LIU Yutong;WU Xianjun;CHEN Xiaoqiong(Rice Research Institute of Sichuan Agricultural University,Chengdu 611130,China;The Crop Gene Resources and Genetic Improvement Key Laboratory of Sichuan Province,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2022年第1期42-49,共8页
Journal of Sichuan Agricultural University
基金
四川省科技厅项目(2021YFYZ0020、2021YFN0043和2020JDJQ0040)
成都市科技局项目(2021-YF05-01700-SN和2021-YF05-02090-SN)。
关键词
稻米
糖尿病
小鼠
血糖
血脂
rice
diabetes
mice
blood sugar
blood lipid
