摘要
目的研究咬合支持改变对幼年大鼠骨骼生长的影响及其与应激有关HPA轴的作用机制。方法4~5周龄雄性SD大鼠,随机分为正常对照组(A组)、单侧上颌磨牙拔除组(B组)、双侧上颌磨牙拔除组(C组),每组6只;记录大鼠术后4、8周体重,ELISA检测大鼠术后4、8周HPA轴及骨代谢相关激素水平,术后8周用HE染色检测股骨骨小梁组织学改变及Micro-CT检测股骨骨小梁骨微结构的改变。结果各组大鼠体重差异无统计学意义。术后4、8周实验组大鼠血浆CRH、ACTH、CORT水平显著升高,BGP水平显著下降趋势。造模8周后实验组股骨骨小梁数量明显减少,骨小梁变细,排列疏松。BV/TV和Tb.Th及TN显著降低,Tb.Sp显著升高。结论幼年大鼠牙齿咬合支持改变使股骨骨小梁形态改变、增加骨质丢失,其机制与脑区HPA轴功能异常影响骨代谢有关。
Objective To study the effects of occlusal disharmony on the growth and development of bone in young rats and the mechanism with HPA axis related to stress.Methods 4-5-week male SD rats were randomly divided into normal control group,unilateral maxillary molar extraction group,and bilateral maxillary molar extraction group,with 6 rats in each group,After 4 and 8 weeks of operation,the levels of HPA axis and bone metabolism related hormones were detected using ELISA.Histological changes of the femoral trabecular were detected with HE staining.The microstructural changes in trabecular bone of the femur were detected with micro-CT after 8 weeks.Results There was no significant difference in body weight among rats in each group.Serum levels of CRH,ACTH,and CORT in the experimental group increased significantly and the BGP level decreased significantly after 4 and 8 weeks.The number of trabeculae of the femoral bone decreased obviously after 8 weeks of modeling.The trabeculae became thinner and loose.BV/TV,Tb.Th,and TN decreased significantly,and Tb.Sp increased significantly.Conclusion The changes of dental occlusal support in juvenile rats result in changes of trabecular bone morphology and increase in bone loss.The mechanism is related to the abnormal HPA axis function in brain region.
作者
刘艳飞
李伯琦
陈朝旭
刘奕杉
LIU Yanfei;LI Boqi;CHEN Zhaoxu;LIU Yishan(Department of Children’s Stomatology-Department of Oral Prevention, the First Affiliated Hospital of Xinjiang Medical University (Affiliated Stomatological Hospital), Urumqi 830054;Institute of Stomatology of Xinjiang Autonomous Region, Urumqi 830054, China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2022年第2期215-219,共5页
Chinese Journal of Osteoporosis
基金
新疆维吾尔自治区自然科学基金(2016D01C316)。
关键词
幼年大鼠
咬合支持改变
应激
HPA轴
骨小梁
young rats
occlusal support changes
stress
HPA axis
trabecular bone
