摘要
运用网络药理学研究方法,对高根二醇作用潜在靶点与机制进行整合与分析,探究高根二醇抗溃疡性结肠炎(UC)作用机制。利用PharmMapper、uniprot对筛选出高根二醇的224个作用靶点,利用OMIM、Genecards数据库检索并筛选出608个疾病作用靶点,整合筛选出高根二醇和溃疡性结肠炎的共同靶点48个。使用Cytoscape 3.7.2软件,构建"成分-靶点-疾病"网络图,再利用STRING、Cytoscape构建PPI网络,筛选出核心靶点,并进行关键靶基因GO与KEGG功能富集分析。根据网络药理学预测的靶点,选择EFGR、IGF1及IL2进行分子对接。本实验初步揭示出高根二醇可能通过抑制炎症反应、调节免疫功能的途径治疗UC。
For uncovering the potential targets and mechanism of erythrodiol in the treatment of Ulcerative Colitis(UC), Network Pharmacology was applied in the study. PharmMapper and Uniprot were used to screen out 224 targets of erythrodiol, OMIM and Genecards database were used to search and screen out 608 disease targets, and 48 common targets of erythrodiol and ulcerative colitis were integrated. Cytoscape 3.7.2 software was applied to construct the “components-targets-disease” network diagram, and STRING and Cytoscape were used to construct PPI network to screen out core targets, and the functional enrichment analysis of key target genes GO and KEGG was conducted. EFGR, IGF1 and IL2 were selected for molecular docking according to the target predicted by network pharmacology. This experiment preliminarily revealed that erythrodiol may treat UC by inhibiting inflammatory response and regulating immune function.
作者
古亮
兰媛
欧阳婷
Gu Liang;Lan Yuan;Ou Yangting(Jiangxi Qingfeng Pharmaceutical Co.,Ltd.,Ganzhou 341000,China)
出处
《广东化工》
CAS
2022年第4期79-81,62,共4页
Guangdong Chemical Industry
关键词
高根二醇
网络药理学
分子对接
成分-靶点-疾病网络
核心靶点
erythrodiol
network pharmacology
molecule docking
components-targets-disease network
the core targets
