摘要
目的:探讨血清miR-1225-5p在骨肉瘤病人诊断及预后中的价值。方法:选取104例骨肉瘤病人(骨肉瘤组)、45例骨膜炎病人(骨膜炎组)和50名健康体检者(对照组)为研究对象。用RT-PCR检测血清中miR-1225-5p的表达水平。以中位数为截断值,将骨肉瘤病人分为高表达组和低表达组。绘制ROC曲线分析miR-1225-5p诊断骨肉瘤的价值;Kaplan-Meier分析和Log-Rank检验分析生存情况;采用COX比例风险回归模型分析影响病人预后的因素。结果:骨肉瘤组血清miR-1225-5p相对表达量均低于骨膜炎组和对照组(P<0.01)。骨肉瘤病人血清miR-1225-5p表达水平与Enneking分期、肿瘤转移有关(P<0.01和P<0.05),而与性别、年龄、肿瘤部位、肿瘤直径和WHO骨肉瘤分类无关(P>0.05)。血清miR-1225-5p诊断骨肉瘤的曲线下面积为0.785(95%CI:0.625~0.934)。当血清miR-1225-5p相对表达量为0.46时约登指数最大,此时灵敏度和特异度分别为89.35%和71.30%。高表达组病人的总生存期和无病生存期均高于低表达组(P<0.05)。血清miR-1225-5p是影响骨肉瘤病人OS和DFS的独立因素(P<0.05和P<0.01)。结论:miR-1225-5p在骨肉瘤的诊断中有重要意义,低血清miR-1225-5p表达水平与骨肉瘤病人的不良预后有关。
Objective:To explore the value of serum miR-1225-5p in the diagnosis and prognosis of osteosarcoma.Methods:One hudred and four patients with osteosarcoma,45 patients with periostitis and 50 healthy people were divided into the osteosarcoma group,periostitis group and control group,respectively.The serum expression levels of miR-1225-5p in three groups were detected using RT-PCR.The median was set as the cutoff value,the osteosarcoma group was subdivided into the high expression group and low expression group.The value of miR-1225-5p in the diagnosis of osteosarcoma was analyzed using ROC curve,Kaplan-Meier analysis and Log-Rank test were used to analyze the survival,and the COX proportional risk regression model was used to analyze the factors affecting the prognosis of patients.Results:The relative expression level of serum miR-1225-5p in osteosarcoma group was lower than that in periostitis group and control group(P<0.01).The expression of miR-1225-5p was correlated with the Enneking stage and tumor metastasis(P<0.01 and P<0.05),but the gender,age,tumor location,tumor diameter were not correlated with the WHO classification of osteosarcoma(P>0.05).The area under the curve of serum miR-1225-5p in the diagnosis of osteosarcoma was 0.785(95%CI:0.625-0.934).When the relative expression of serum miR-1225-5p was 0.46,the Yoden index was the highest,and the sensitivity and specificity were 89.35%and 71.30%,respectively.The total survival time and disease-free survival time in high expression group were higher than those in low expression group(P<0.05).The serum miR-1225-5p was an independent factor of OS and DFS in patients with osteosarcoma(P<0.05 and P<0.01).Conclusions:MiR-1225-5p plays an important role in the diagnosis of osteosarcoma.The low expression level of serum miR-1225-5p is related to the poor prognosis of osteosarcoma patients.
作者
李伟
汪礼军
唐鹏
黄耀凯
刘剑
LI Wei;WANG Li-jun;TANG Peng;HUANG Yao-kai;LIU Jian(Department of Orthopaedics,Chongqing Dazu District People′s Hospital,Chongqing 402360,China)
出处
《蚌埠医学院学报》
CAS
2022年第2期176-180,共5页
Journal of Bengbu Medical College
基金
重庆市卫生计生委医学科研项目(2017ZBXM041)。
