摘要
【目的】结合广西防城港海区的水质和环境条件,探讨影响牟氏角毛藻生长的主要理化因子,筛选最佳理化参数,为规模化扩培生产提供技术数据。【方法】采用单因子变量方法研究盐度、温度、光照强度对牟氏角毛藻生长的影响,其中盐度设置0‰、5‰、10‰、20‰、30‰、35‰、40‰共7个梯度,温度设置25、28、31、34℃共4个梯度,光照强度设置1000、4000、7000 lx共3个梯度。【结果】牟氏角毛藻在盐度5‰~40‰条件下均能正常生长,20‰盐度为牟氏角毛藻的最适盐度;高温培养条件下,31℃时牟氏角毛藻达到最大藻细胞浓度;温度越低,生长速度越慢,稳定期越长;温度越高,生长速度越快,稳定期越短;4000 lx为牟氏角毛藻的最适生长光照强度,光照强度越高,稳定期越长。【结论】20‰盐度、31℃水温和4000 lx光照强度是广西防城港海区牟氏角毛藻的最佳生长条件,生长速度和藻细胞密度均达最高值;过高或过低的盐度、温度、光照强度均会抑制牟氏角毛藻的生长。
【Objective】Combining with the seawater environmental conditions in Fangchenggang sea area along the coast of Guangxi,the main physical and chemical factors of the growth of Chaetoceros muelleri was explored and the optimal growth environment conditions were screened out in order to provide technical data for large-scale production.【Method】The effects of salinity,temperature and light on the growth of C.muelleri were studied by single factor method.The experimental design was as follows:seven gradients of 0‰,5‰,10‰,20‰,30‰,35‰and 40‰were set for salinity test,four gradients of 25,28,31 and 34℃were set for temperature test,and three gradients of 1000,4000 and 7000 lx were set for the light intensity.【Result】C.muelleri could grow normally with the salinity of 5‰-40‰,among which the salinity of 20‰was the optimum growth condition.Under the condition of high temperature culture,the cell concentration of C.muelleri reached the maximum at 31℃.The lower the temperature,the slower the growth rate,the longer the stable period;the higher the temperature,the faster the growth rate and the shorter the stable period.4000 lx was the most suitable light intensity for the growth of C.muelleri.The higher the light intensity,the longer the stability period.【Conclusion】The best growth conditions for C.muelleri in Fangchenggang sea area along the coast of Guangxi were:20‰salinity,31℃temperature and 4000 lx light intensity,Under such conditions,both the growth rate and algal cell density reached the highest values.Excessive or low salinity,temperature and light could inhibit the growth of C.muelleri.
作者
朱昔恩
林原有
项桂德
熊建华
陈田聪
陈晓汉
张彬
ZHU Xien;LIN Yuanyou;XIANG Guide;XIONG Jianhua;CHEN Tiancong;CHEN Xiaohan;ZHANG Bin(Guangxi Academy of Fisheries Science/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning 530021,China)
出处
《广东农业科学》
CAS
2022年第1期136-141,共6页
Guangdong Agricultural Sciences
基金
国家现代农业产业技术体系专项(CARS-48)
国家现代农业产业技术体系广西对虾产业创新团队建设专项(nycytxgxcxtd-14-03)
广西科技重大专项广西创新驱动发展专项(桂科AA17204094-1)
西南野生动植物资源保护教育部重点实验室开放基金(XNYB17-6)。
关键词
牟氏角毛藻
盐度
温度
光照
藻细胞密度
生长速率
Chaetoceros muelleri
salinity
temperature
light
algal cell density
growth rate
