摘要
目的探讨重度子痫前期(SPE)孕妇脐动脉平滑肌中经典瞬时受体电位通道1(TRPC1)的表达及意义。方法选取2020年1—8月在西南医科大学附属医院产科确诊为SPE和慢性高血压并发SPE(CHPE)的单胎孕妇各15例为研究对象,分别设为SPE组和CHPE组,另选取同期正常健康晚孕单胎孕妇15例为对照组。采用荧光定量PCR(qPCR)与蛋白质印迹技术分别检测3组孕妇脐动脉平滑肌中TRPC1的表达。结果SPE组与CHPE组脐动脉平滑肌中TRPC1的mRNA及蛋白表达水平均比对照组高,差异有统计学意义(P<0.05);但SPE组与CHPE组组间比较,差异无统计学意义(P>0.05)。结论TRPC1在CHPE及SPE孕妇脐动脉平滑肌中表达上调,提示SPE疾病的发生发展可能与TRPC1的过表达有关。
Objective To investigate the expression and significance of transient receptor potential canonical 1(TRPC1)in human umbilical artery smooth muscle in severe preeclampsia(SPE).Methods Singleton pregnant women who were diagnosed with SPE or chronic hypertension complicated with severe preeclampsia(CHPE)in the obstetrics department of the Affiliated Hospital of Southwest Medical University from January to August of 2020 were selected as the research objects.They were divided into SPE group(n=15)and CHPE group(n=15).And another 15 healthy singleton pregnant women in the same period were selected as the control group.The expression of TRPC1 in umbilical artery smooth muscle cells was detected by real-time fluorescent quantitative PCR(qPCR)and Wertern blot.Results The mRNA and protein expression levels of TRPC1 in the umbilical artery smooth muscle of pregnant women in SPE group and CHPE group were higher than those in the control group,and the differences were statistically significant(P<0.05),but there was no significant difference between SPE group and CHPE group(P>0.05).Conclusion TRPC1 expression is up-regulated in CHPE and SPE pregnant women,suggesting that the occurrence and development of SPE disease may be related to the overexpression of TRPC1.
作者
袁德丽
周清
傅晓冬
Yuan Deli;Zhou Qing;Fu Xiaodong(Department of Obstetrics, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China;Central Laboratory of the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China)
出处
《成都医学院学报》
CAS
2022年第1期12-15,共4页
Journal of Chengdu Medical College
基金
四川省科技厅2012年度第三批科技计划项目(科技支撑计划)(No:2012SZ0178)。
关键词
重度子痫前期
人脐动脉平滑肌
经典瞬时受体电位通道1
Severe preeclampsia
Human umbilical artery smooth muscle
Transient receptor potential canonical 1
