摘要
应用正交试验法对金铁锁愈伤组织诱导与芽分化离体快繁培养基进行优化。针对温度、6-BA、2,4-D、蔗糖等关键因子,用L_(9)(3^(4))表安排4因素3水平的正交试验。经方差分析后得出6-BA(1.5 mg/L)+2,4-D(0.5 mg/L)+蔗糖(30 g/L)+温度(21℃)是诱导愈伤组织的较优的培养基。光照1600 lx、光周期12 h最为优化组织培养条件。
The culture medium of callus induction and bud differentiation in vitro rapid propagation was optimized by orthogonal test.According to the key factors such as temperature,6-BA,2,4-D and sucrose,the orthogonal test of 4 factors and 3 levels was arranged with L_(9)(3^(4)).The results showed that 6-BA(1.5 mg/L)+2,4-D(0.5 mg/L)+sucrose(30 g/L)+temperature(21℃)was the optimal medium for callus induction.Light 1600 lx and photoperiod 12 h were the most optimized tissue culture conditions.
作者
张丽霞
Zhang Lixia(Shuozhou Vocational and Technical College,Shuozhou 038500,Shanxi,China)
出处
《农业技术与装备》
2021年第12期29-29,32,共2页
Agricultural Technology & Equipment
关键词
正交试验
金铁锁
方差分析
orthogonal test
Psammosilene tunicoides
analysis of variance
