摘要
目的基于动物实验和网络药理学研究白竭散保留灌肠治疗溃疡性结肠炎(UC)的作用机制。方法将42只SD大鼠随机抽出10只为空白组,剩余32只大鼠通过5%2,4,6-三硝基苯磺酸溶液(TNBS)灌肠法构建UC大鼠模型。在32只大鼠中随机抽出2只,麻醉处死,剪取距肛门约8 cm肠道,显微镜下观察结肠组织,见黏膜层炎症改变,溃疡形成,造模成功。余鼠30只按照随机数字表法分为3组,模型组、美沙拉秦组、白竭散组,每组10只。造模成功后次日,白竭散组予白竭散混悬液2.3 g/(kg•d)灌肠,美沙拉秦组予美沙拉秦灌肠液0.7 g/(kg•d)灌肠,模型组、空白组予0.9%氯化钠注射液2 mL灌肠。观察各组大鼠治疗后症状表现及结肠组织损伤情况;比较各组大鼠疾病活动指数(DIA)、结肠黏膜损伤指数(CMDI)评分变化;观察各组大鼠结肠组织苏木精-伊红(HE)染色结果。通过相关数据库提取白竭散有效成分和靶点、UC疾病靶点基因,构建“药物-有效成分-疾病靶点”网络图、蛋白质-蛋白质相互作用(PPI)网络,进行基因本体生物学过程(GOBP)及京都基因与基因组百科全书(KEGG)通路富集分析,挖掘其作用机制。结果治疗后空白组大鼠体质量无明显变化,活动量正常,精神反应灵敏,大便质软成形,无黏液脓血便;模型组大鼠活动量减少,体质量减轻,大便不成形,见脓血便;美沙拉秦组及白竭散组大鼠精神良好,反应尚灵敏,体质量基本正常,大便轻度松散,无黏液脓血便。治疗后空白组大鼠结肠壁结构完整,无损伤;模型组大鼠结肠壁可见多处溃疡,大小不等,最大约1.1 cm,炎症较重;美沙拉秦组及白竭散组大鼠结肠壁溃疡面较少,面积较小,炎性反应轻。模型组大鼠DAI及CMDI评分均高于空白组(P<0.05);美沙拉秦组、白竭散组大鼠DAI及CMDI评分均低于模型组(P<0.05);美沙拉秦组与白竭散组大鼠DAI及CMDI评分比较差异无统计学意义(P>0.05)。空白组�
Objective Mechanism of Baijie Powder retention enema for ulcerative colitis(UC)based on animal experiments and network pharmacology mining.Methods Ten of 42 SD rats were randomly selected as blank group,and the remaining 32 rats were given enema intervention of 2,4,6-trinitrobenzenesulfonic acid(TNBS,concentration 5%)to establish UC rat model.Two of the 32 rats were randomly sacrificed by anesthesia,and then the intestinal tract about 8 cm away from the anus was obtained observing the colon tissue under the microscope;when mucosal inflammation changes and ulcer formation were observed,here,the model was successfully established.The next day,a random number table method make 30 rats into 3 groups:model group,mesalazine group[0.7 g/(kg•d),once daily(QD)]and Baijie Powder group[2.3 g/(kg•d),QD],with 10 rats in each group,model group and blank group were given 2 mL of 0.9%sodium chloride injection to enema.Posttreatment symptoms and colic injury of rats in each group were observed.The key observation was disease activity index(DIA)and colonic mucosal injury index(CMDI)and HE staining results of colon tissue in groups.The active ingredients and targets of Baijie Powder and UC disease targets were extracted in relevant databases,a"drug-active ingredient-disease target"network diagram and a"protein-protein interaction(PPI)"network were built,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis was performed,exploring their mechanisms of action.Results After treatment,the rats in the blank group had no significant changes in body weight,normal activity levels,sensitive responses,soft-formed stools,and no mucus,pus and blood in the stools;the rats in the model group had reduced activity,decreased body weight,unformed stools,and pus and blood stools in the stools;posttreatment symptoms similar to blank group were found in Rats of the mesalazine group and Baijie Powder group.After treatment,the structure of intestine wall of the rats in the blank group was complete,and with no damage;the colon wall o
作者
赵梦月
孙晓健
王宏昌
毛细云
ZHAO Mengyue;SUN Xiaojian;WANG Hongchang;MAO Xiyun(Graduate School of Anhui University of Chinese Medicine,Hefei,Anhui 230012;不详)
出处
《河北中医》
2021年第11期1866-1873,1932,共9页
Hebei Journal of Traditional Chinese Medicine
基金
安徽中医药大学2019年度国家重点专科(中医外科)开放性课题(编号:2019zdzk06)。
关键词
灌肠
结肠炎
溃疡性
大鼠
白竭散
网络药理学
中药药理学
Enema
Colitis,ulcerative
Rat
Baijie Powder
Network pharmacology
Pharmacology of Chinese medicine
