摘要
目的阐明高频重复经颅磁刺激(rTMS)对脑梗死大鼠认知功能的影响,通过RNA测序探索其潜在机制。方法选择30只SD雄性大鼠进行短暂性大脑中动脉闭塞(tMCAO)造模,根据Bederson评分排除10只不在1~3分的大鼠,剩余20只模型大鼠用随机数字表法分为tMCAO组(n=10)和rTMS组(n=10),另设假手术组(n=10)。rTMS组大鼠于术后第7~28天接受20 Hz rTMS干预治疗。术后第28~33天进行Morris水迷宫实验检测各组大鼠认知功能,在rTMS组和tMCAO组各取3只大鼠的梗死灶周围皮质组织进行RNA测序。结果rTMS组[(53±4)s]和假手术组[(51±5)s]的逃避潜伏期明显短于tMCAO组[(58±4)s,P<0.05];rTMS组[2.5(1.5~3.0)次]和假手术组[3.0(1.5~3.0)次]大鼠在60 s内穿越原平台的次数多于tMCAO组[1.0(0.5~1.5)次,P<0.05]。RNA测序共发现16个显著差异表达基因,包括9个上调基因和7个下调基因。GO分析结果显示,上调基因的功能主要集中于化学和金属离子稳态等过程,而下调基因的功能则主要集中于炎症反应。结论20 Hz rTMS能够显著改善脑梗死大鼠的认知功能,其机制可能与维持化学和金属离子稳态及调控小胶质细胞的极化减轻神经炎症相关。
Objective To clarify the effect of high-frequency repetitive transcranial magnetic stimulation(rTMS)on cognitive function in cerebral ischemic rats,and to explore its underlying mechanism by RNA sequencing.Methods Thirty male Sprague-Dawley(SD)rats underwent transient middle cerebral artery occlusion(tMCAO).According to the Bederson score,10 rats with a score of 1-3 were excluded,and the remaining 20 rats were then randomly divided into the tMCAO group(n=10)and the rTMS group(n=10).Meanwhile,10 rats with sham operation were assigned to the sham group(n=10).Rats in the rTMS group received 20 Hz rTMS from day 7 to day 28 after surgery.From day 28 to day 33 after the operation,Morris water maze test was performed to detect the cognitive function of rats in each group.The cortical tissues around the infarcts from the rTMS tMCAO groups were taken for RNA sequencing analysis,with 3 rats in each group.Results The escape latency of rats in the rTMS group[(53±4)s]and the group[(51±5)s]were significantly shorter than that of the tMCAO group[(58±4)s,P<0.05)].The times that the rats crossed the original platform in 60 seconds in the rTMS group[2.5(1.5-3.0)]and sham group[3.0(1.5-3.0)]were more than that of the tMCAO group[1.0(0.5-1.5)](P<0.05).RNA sequencing detected 16 significantly differentially expressed genes,including 9 up-regulated genes and 7 down-regulated genes.GO analysis showed that the functions of up-regulated genes were mainly concentrated in the processes of chemical homeostasis and cell metal ion homeostasis.While the functions of down-regulated genes mainly enriched in the inflammatory response.Conclusion Twenty Hz rTMS can improve the cognitive function of rats with cerebral infarction,and its underlying mechanism may be related to maintaining chemical and metal ion homeostasis and regulating the polarization of microglia to reduce neuroinflammation.
作者
洪洁娜
陈洁梅
曾妍
李超
张雪
贺子桐
温红梅
Hong Jiena;Chen Jiemei;Zeng Yan;Li Chao;Zhang Xue;He Zitong;Wen Hongmei(Department of Rehabilitation Medicine,the Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China;Department of Rehabilitation Medicine,Meizhou People′s Hospital,Meizhou 514000,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2022年第1期73-79,共7页
National Medical Journal of China
基金
国家自然科学基金(81672259,81472156)
广东省基础与应用基础研究基金(2019A1515010388)。
关键词
经颅磁刺激
短暂性大脑中动脉闭塞
RNA测序
认知功能
Transcranial magnetic stimulation
Transient middle cerebral artery occlusion
RNA sequencing
Cognitive function
